Detailed information of TA system TA123215

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF2/-
• Location: 370483..371001
• Replicon: chromosome
• Accession: NZ_CP038819
• Organism: Staphylococcus aureus strain O82

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: SaO82_RS01710
• Protein ID: WP_000448764.1
• Coordinates: 370699..371001 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF2
Locus tag	-
Coordinates	370483..370619 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO82_RS01670	365577..365867	+	291	WP_000179860.1	hypothetical protein	-
SaO82_RS01675	365883..367793	+	1911	WP_136142511.1	hypothetical protein	-
SaO82_RS01680	367793..369259	+	1467	WP_000067157.1	BppU family phage baseplate upper protein	-
SaO82_RS01685	369259..369648	+	390	WP_001166604.1	DUF2977 domain-containing protein	-
SaO82_RS01690	369641..369805	+	165	WP_000916020.1	XkdX family protein	-
SaO82_RS01695	369851..370150	+	300	WP_000466773.1	DUF2951 family protein	-
SaO82_RS14050	370302..370491	+	190	Protein_326	putative holin-like toxin	-
-	370483..370619	-	137	NuclAT_0	-	Antitoxin
-	370483..370619	-	137	NuclAT_0	-	Antitoxin
-	370483..370619	-	137	NuclAT_0	-	Antitoxin
-	370483..370619	-	137	NuclAT_0	-	Antitoxin
SaO82_RS01705	370538..370648	-	111	WP_070003492.1	hypothetical protein	-
SaO82_RS01710	370699..371001	+	303	WP_000448764.1	phage holin	Toxin
SaO82_RS01715	371013..372469	+	1457	Protein_329	N-acetylmuramoyl-L-alanine amidase	-
SaO82_RS01725	373982..374479	+	498	WP_136142512.1	hypothetical protein	-
SaO82_RS01735	375039..375866	-	828	WP_136142513.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	328541..372469	43928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11230.99 Da
• Isoelectric Point: 9.6587

>T123215 WP_000448764.1 NZ_CP038819:370699-371001 [Staphylococcus aureus]
METKVITRYIVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTAYKDNPTSQEGRWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T123215 NZ_CP038819:370699-371001 [Staphylococcus aureus]
ATGGAAACAAAAGTAATAACAAGATATATTGTATTAATTTTAGCGTTAGTCAATCAGTTTTTAGCGAACAAAGGTATAAG
CCCGATACCAGTAGATGAAGAGAGTATTTCATCGATTATCTTAACAGTCATTGCTTTATACACCGCTTATAAAGACAATC
CAACATCACAAGAAGGACGCTGGGCGAATCAAAAACTAAAGAAATATAAAGCTGAAAATAAGTATAGAAAAGCAACAGGA
CAAGCGCCAATTAAAGAAGTAATGACACCTACAAATATGAATGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 137 bp

>AT123215 NZ_CP038819:c370619-370483 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGACCTCTGTAGTTAAAT
GAATTTATATAATCCTCTAACCATCGTACTCGTCAAAGTTAGCGATGGTTATTTTTT

Structures

Antitoxin