Detailed information of TA system TA123214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2070958..2071627
• Replicon: chromosome
• Accession: NZ_CP038817
• Organism: Haemophilus parahaemolyticus strain NEB129

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I3DML4
• Locus tag: E5Q53_RS10090
• Protein ID: WP_005705805.1
• Coordinates: 2071268..2071627 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E5Q53_RS10085
• Protein ID: WP_039863038.1
• Coordinates: 2070958..2071257 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Q53_RS10045	2066204..2067217	+	1014	WP_005705634.1	hypothetical protein	-
E5Q53_RS10050	2067271..2067672	+	402	WP_005705631.1	hypothetical protein	-
E5Q53_RS10055	2067681..2068013	+	333	WP_039862990.1	hypothetical protein	-
E5Q53_RS10060	2068013..2068339	+	327	WP_005705626.1	phage tail protein	-
E5Q53_RS10065	2068336..2069049	+	714	WP_005705787.1	phage minor tail protein L	-
E5Q53_RS10070	2069170..2069400	+	231	WP_005705685.1	hypothetical protein	-
E5Q53_RS10075	2069401..2070159	+	759	WP_005705809.1	hypothetical protein	-
E5Q53_RS10080	2070218..2070961	+	744	WP_039863041.1	C40 family peptidase	-
E5Q53_RS10085	2070958..2071257	-	300	WP_039863038.1	helix-turn-helix transcriptional regulator	Antitoxin
E5Q53_RS10090	2071268..2071627	-	360	WP_005705805.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5Q53_RS10095	2071716..2072282	+	567	WP_005705652.1	tail assembly protein	-
E5Q53_RS10100	2072325..2072540	-	216	WP_005705669.1	BrnA antitoxin family protein	-
E5Q53_RS10105	2072537..2072827	-	291	WP_005705812.1	BrnT family toxin	-
E5Q53_RS10110	2072904..2073143	+	240	Protein_1936	hypothetical protein	-
E5Q53_RS10115	2073180..2073938	-	759	WP_078208072.1	IS3 family transposase	-
E5Q53_RS10120	2074002..2074928	+	927	WP_005706750.1	hypothetical protein	-
E5Q53_RS10125	2075153..2075866	+	714	WP_005706757.1	UMP kinase	-
E5Q53_RS10130	2075912..2076469	+	558	WP_005706753.1	ribosome recycling factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	msbA	1959533..2079623	120090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-84)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 14034.12 Da
• Isoelectric Point: 9.5949

>T123214 WP_005705805.1 NZ_CP038817:c2071627-2071268 [Haemophilus parahaemolyticus]
MKKQSQWTVIATERFNLWLLEQTEDVRQSVFASLNNLKMYGYQLSRPYADTIKGSKYPNMKELRIQHKGKPLRAFFAFDP
LRQAIVLCAGDKSNDKQFYERMIKIADAEFTDYLQSLEQ

Nucleotide

• Length: 360 bp

>T123214 NZ_CP038817:c2071627-2071268 [Haemophilus parahaemolyticus]
ATGAAAAAACAATCTCAATGGACGGTAATCGCTACTGAACGATTTAATCTCTGGCTGCTTGAGCAAACCGAAGATGTTCG
TCAATCGGTGTTTGCATCGCTCAATAACTTGAAAATGTATGGTTACCAGCTTTCACGTCCTTATGCTGACACGATAAAAG
GCTCAAAATATCCGAATATGAAAGAACTTCGCATTCAGCATAAAGGCAAGCCGTTGCGAGCGTTTTTCGCTTTCGACCCA
CTTAGACAAGCTATTGTGCTCTGTGCGGGGGATAAAAGTAACGACAAACAATTTTATGAGCGAATGATTAAAATCGCCGA
TGCAGAATTTACCGACTACTTACAAAGTTTGGAGCAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11078.86 Da
• Isoelectric Point: 4.6538

>AT123214 WP_039863038.1 NZ_CP038817:c2071257-2070958 [Haemophilus parahaemolyticus]
MYTLEEMIARETPESQQKIKEMSDELILETGLAMIRENLALSQKEMAQAVGVSQSAIAQIEQRGNDVKLSTLKRYIEKMG
GELSLAVKMPTGYSQIFPI

Nucleotide

• Length: 300 bp

>AT123214 NZ_CP038817:c2071257-2070958 [Haemophilus parahaemolyticus]
ATGTACACCCTTGAAGAGATGATTGCCCGTGAAACACCTGAGTCTCAACAAAAAATCAAAGAGATGTCCGATGAACTGAT
TTTAGAAACAGGGCTTGCGATGATTCGTGAAAACCTAGCATTGTCGCAAAAAGAGATGGCTCAAGCCGTAGGTGTATCTC
AATCCGCGATTGCTCAGATTGAACAGCGTGGCAATGATGTTAAACTCTCTACACTTAAACGCTACATTGAGAAAATGGGC
GGTGAACTCAGCCTTGCCGTCAAAATGCCAACAGGTTACAGCCAGATTTTTCCAATTTAA

Structures

Toxin

• AlphaFold DB: I3DML4