Detailed information of TA system TA123209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1054074..1054726
• Replicon: chromosome
• Accession: NZ_CP038817
• Organism: Haemophilus parahaemolyticus strain NEB129

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I3DHX3
• Locus tag: E5Q53_RS04885
• Protein ID: WP_005706111.1
• Coordinates: 1054074..1054421 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E5Q53_RS04890
• Protein ID: WP_005706149.1
• Coordinates: 1054418..1054726 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Q53_RS04865	1050174..1051241	+	1068	WP_039863143.1	UDP-N-acetylglucosamine--undecaprenyl-phosphate N-acetylglucosaminephosphotransferase	-
E5Q53_RS04870	1051304..1052080	+	777	WP_005706138.1	hypothetical protein	-
E5Q53_RS04875	1052254..1052487	-	234	WP_005706133.1	hypothetical protein	-
E5Q53_RS04880	1052630..1053967	+	1338	WP_005706142.1	ATP-dependent RNA helicase SrmB	-
E5Q53_RS04885	1054074..1054421	+	348	WP_005706111.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5Q53_RS04890	1054418..1054726	+	309	WP_005706149.1	helix-turn-helix domain-containing protein	Antitoxin
E5Q53_RS04895	1054769..1055326	+	558	WP_005706141.1	DUF805 domain-containing protein	-
E5Q53_RS04900	1055449..1056033	+	585	WP_005706115.1	aminoacyl-tRNA hydrolase	-
E5Q53_RS04905	1056211..1057302	+	1092	WP_005706109.1	redox-regulated ATPase YchF	-
E5Q53_RS04910	1057473..1058486	+	1014	WP_005706121.1	tRNA pseudouridine(13) synthase TruD	-
E5Q53_RS04915	1058494..1059270	+	777	WP_005706145.1	5'/3'-nucleotidase SurE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (11-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-86)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13389.30 Da
• Isoelectric Point: 10.1667

>T123209 WP_005706111.1 NZ_CP038817:1054074-1054421 [Haemophilus parahaemolyticus]
MKLTFIELPPFERYRKEHISDEAYRLFQNELLDNPEKGDVIQGTGGLRKIRLADHSRHKGKRGGARAIYHYFVNGNQIWL
FAVYGKGEKEDLNETEKRTFKVVLENLKALARGNK

Nucleotide

• Length: 348 bp

>T123209 NZ_CP038817:1054074-1054421 [Haemophilus parahaemolyticus]
ATGAAATTAACTTTTATCGAATTACCACCCTTTGAACGATATCGAAAAGAACATATTAGTGATGAAGCGTATCGACTTTT
CCAAAATGAACTATTAGATAATCCAGAGAAAGGTGATGTTATTCAAGGAACAGGCGGGTTAAGAAAAATTCGATTAGCTG
ATCATTCTCGTCATAAAGGTAAACGAGGAGGAGCAAGGGCAATTTACCATTATTTTGTTAATGGTAATCAGATTTGGCTT
TTTGCCGTTTATGGAAAAGGCGAAAAAGAGGATTTAAACGAAACGGAAAAAAGAACGTTTAAAGTTGTTTTAGAAAACTT
AAAAGCATTAGCAAGGGGGAATAAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11528.28 Da
• Isoelectric Point: 9.8116

>AT123209 WP_005706149.1 NZ_CP038817:1054418-1054726 [Haemophilus parahaemolyticus]
MSRLFDDLMEGAKALEDHLQGKITLRTETLEKSEPVTISANEVKAIREKLNLSQAVFAHRLRTSVRTYQGWEQGKTKPNP
QAALLLRMVEHSPQTFNLIVGV

Nucleotide

• Length: 309 bp

>AT123209 NZ_CP038817:1054418-1054726 [Haemophilus parahaemolyticus]
ATGAGCCGTTTATTTGACGATTTAATGGAAGGGGCAAAAGCACTCGAAGATCACTTACAAGGCAAAATCACATTACGCAC
CGAGACGCTTGAAAAATCAGAGCCTGTGACGATCAGTGCCAACGAAGTGAAAGCTATTCGTGAAAAATTAAATTTATCTC
AAGCAGTATTTGCTCATCGTTTGAGAACAAGCGTGAGAACCTATCAAGGTTGGGAGCAAGGAAAAACAAAACCGAATCCA
CAAGCCGCATTATTATTAAGAATGGTTGAACATTCACCGCAAACATTTAATTTAATCGTTGGCGTATAA

Structures

Toxin

• AlphaFold DB: I3DHX3