Detailed information of TA system TA123163

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64601..64855
• Replicon: plasmid pDW54_1
• Accession: NZ_CP038792
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: E5M00_RS26395
• Protein ID: WP_047649939.1
• Coordinates: 64601..64807 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	64794..64855 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS26350	60117..60884	+	768	WP_001159649.1	DUF1883 domain-containing protein	-
E5M00_RS26355	61252..61527	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E5M00_RS26360	61527..61811	-	285	WP_024187170.1	ribbon-helix-helix protein, CopG family	-
E5M00_RS26375	62898..63755	-	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
E5M00_RS26380	63748..63822	-	75	WP_001365705.1	RepA leader peptide Tap	-
E5M00_RS26390	64069..64317	-	249	WP_021512942.1	replication regulatory protein RepA	-
E5M00_RS26395	64601..64807	-	207	WP_047649939.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	64794..64855	+	62	NuclAT_0	-	Antitoxin
-	64794..64855	+	62	NuclAT_0	-	Antitoxin
-	64794..64855	+	62	NuclAT_0	-	Antitoxin
-	64794..64855	+	62	NuclAT_0	-	Antitoxin
E5M00_RS26405	65030..65412	-	383	Protein_74	hypothetical protein	-
E5M00_RS26410	65515..65976	-	462	WP_000760078.1	thermonuclease family protein	-
E5M00_RS26415	66719..66922	-	204	WP_001327131.1	hypothetical protein	-
E5M00_RS26420	67077..67634	-	558	WP_000139312.1	fertility inhibition protein FinO	-
E5M00_RS26425	67737..68597	-	861	WP_000704512.1	alpha/beta hydrolase	-
E5M00_RS26430	68656..69402	-	747	WP_000205745.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	espP / faeJ / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / iutA / iucD / iucC / iucB / iucA	1..129920	129920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7762.27 Da
• Isoelectric Point: 8.8807

>T123163 WP_047649939.1 NZ_CP038792:c64807-64601 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGGLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T123163 NZ_CP038792:c64807-64601 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGGGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT123163 NZ_CP038792:64794-64855 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin