Detailed information of TA system TA123155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4062234..4062928
• Replicon: chromosome
• Accession: NZ_CP038791
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: E5M00_RS20365
• Protein ID: WP_001263493.1
• Coordinates: 4062234..4062632 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: E5M00_RS20370
• Protein ID: WP_032162515.1
• Coordinates: 4062635..4062928 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS20335	4057234..4058478	-	1245	WP_000189541.1	esterase FrsA	-
-	4057894..4057974	-	81	NuclAT_11	-	-
-	4057894..4057974	-	81	NuclAT_11	-	-
-	4057894..4057974	-	81	NuclAT_11	-	-
-	4057894..4057974	-	81	NuclAT_11	-	-
E5M00_RS20340	4058570..4059028	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E5M00_RS20345	4059289..4060746	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
E5M00_RS20350	4060803..4061324	-	522	Protein_3887	peptide chain release factor H	-
E5M00_RS20355	4061323..4061526	-	204	Protein_3888	RNA ligase RtcB family protein	-
E5M00_RS20360	4061772..4062224	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
E5M00_RS20365	4062234..4062632	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E5M00_RS20370	4062635..4062928	-	294	WP_032162515.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E5M00_RS20375	4062980..4064035	-	1056	WP_032162516.1	DNA polymerase IV	-
E5M00_RS20380	4064106..4064891	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
E5M00_RS20385	4064836..4066575	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
E5M00_RS20390	4066799..4067296	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T123155 WP_001263493.1 NZ_CP038791:c4062632-4062234 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T123155 NZ_CP038791:c4062632-4062234 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11261.86 Da
• Isoelectric Point: 5.9050

>AT123155 WP_032162515.1 NZ_CP038791:c4062928-4062635 [Escherichia coli]
MHRILAEKSVNITELRKNPVKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT123155 NZ_CP038791:c4062928-4062635 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGTTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8