Detailed information of TA system TA123139

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3031890..3032261
• Replicon: chromosome
• Accession: NZ_CP038791
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: E5M00_RS15055
• Protein ID: WP_021500490.1
• Coordinates: 3032067..3032261 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3031890..3032068 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS15030	3027845..3029218	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
E5M00_RS15035	3029347..3030282	-	936	WP_001157381.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E5M00_RS15040	3030334..3031569	-	1236	WP_000040838.1	site-specific integrase	-
E5M00_RS15045	3031571..3031786	-	216	WP_000079604.1	excisionase XisR	-
E5M00_RS15050	3031886..3032074	-	189	WP_001298826.1	DUF1187 family protein	-
-	3031890..3032068	+	179	NuclAT_0	-	Antitoxin
-	3031890..3032068	+	179	NuclAT_0	-	Antitoxin
-	3031890..3032068	+	179	NuclAT_0	-	Antitoxin
-	3031890..3032068	+	179	NuclAT_0	-	Antitoxin
E5M00_RS15055	3032067..3032261	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E5M00_RS15060	3032325..3033377	-	1053	WP_001004422.1	recombinase RecT	-
E5M00_RS15065	3033389..3036511	-	3123	WP_135798769.1	exodeoxyribonuclease VIII	-
E5M00_RS15070	3036613..3036887	-	275	Protein_2863	hypothetical protein	-
E5M00_RS15075	3036962..3037132	-	171	WP_077688178.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3030334..3078249	47915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T123139 WP_021500490.1 NZ_CP038791:c3032261-3032067 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T123139 NZ_CP038791:c3032261-3032067 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT123139 NZ_CP038791:3031890-3032068 [Escherichia coli]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin