Detailed information of TA system TA123115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4664215..4664945
• Replicon: chromosome
• Accession: NZ_CP038662
• Organism: Serratia nematodiphila strain DH-S01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: E5D60_RS22225
• Protein ID: WP_004937033.1
• Coordinates: 4664215..4664526 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E5D60_RS22230
• Protein ID: WP_004937031.1
• Coordinates: 4664523..4664945 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5D60_RS22195	4659542..4660267	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
E5D60_RS22200	4660320..4661753	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
E5D60_RS22205	4661777..4662064	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
E5D60_RS22210	4662220..4662681	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
E5D60_RS22215	4662809..4663663	+	855	WP_015379177.1	RNase adapter RapZ	-
E5D60_RS22220	4663660..4663932	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
E5D60_RS22225	4664215..4664526	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
E5D60_RS22230	4664523..4664945	+	423	WP_004937031.1	helix-turn-helix domain-containing protein	Antitoxin
E5D60_RS22235	4664950..4666866	-	1917	WP_033632012.1	BglG family transcription antiterminator	-
E5D60_RS22240	4666918..4668132	-	1215	WP_135638684.1	lactonase family protein	-
E5D60_RS22245	4668220..4668960	-	741	WP_033632010.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T123115 WP_004937033.1 NZ_CP038662:4664215-4664526 [Serratia nematodiphila]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T123115 NZ_CP038662:4664215-4664526 [Serratia nematodiphila]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAACAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT123115 WP_004937031.1 NZ_CP038662:4664523-4664945 [Serratia nematodiphila]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQHIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT123115 NZ_CP038662:4664523-4664945 [Serratia nematodiphila]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTCGAATTGGTGGAATACCTGCTGGAAAACGATGAACGCCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCATATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A8B4GR63