Detailed information of TA system TA123113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4190827..4191479
• Replicon: chromosome
• Accession: NZ_CP038662
• Organism: Serratia nematodiphila strain DH-S01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E5D60_RS19950
• Protein ID: WP_048324788.1
• Coordinates: 4190827..4191171 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A086GAN0
• Locus tag: E5D60_RS19955
• Protein ID: WP_004931828.1
• Coordinates: 4191177..4191479 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5D60_RS19940	4187169..4189427	-	2259	WP_135638586.1	molybdopterin guanine dinucleotide-containing S/N-oxide reductase	-
E5D60_RS19945	4189648..4190667	+	1020	WP_135638588.1	HTH-type transcriptional regulator GalR	-
E5D60_RS19950	4190827..4191171	+	345	WP_048324788.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5D60_RS19955	4191177..4191479	+	303	WP_004931828.1	helix-turn-helix transcriptional regulator	Antitoxin
E5D60_RS19960	4191507..4192769	-	1263	WP_060560144.1	diaminopimelate decarboxylase	-
E5D60_RS19965	4192903..4193826	+	924	WP_021505677.1	LysR family transcriptional regulator	-
E5D60_RS19970	4193854..4194762	-	909	WP_085058844.1	LysR family transcriptional regulator	-
E5D60_RS19975	4194871..4195755	+	885	WP_004931820.1	MBL fold metallo-hydrolase	-
E5D60_RS19980	4195830..4196477	+	648	WP_033632219.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13330.40 Da
• Isoelectric Point: 10.0507

>T123113 WP_048324788.1 NZ_CP038662:4190827-4191171 [Serratia nematodiphila]
MWQVVTVERFDDWFLALNNAQQTSILAAIYKLQTFGPQLARPHADTLHFSDAVRQLKELRIQHRGCPFRVFFAFDPQRQA
VLLCGGDKTGDKRFYQRMLPIAAMEFSHYLATRR

Nucleotide

• Length: 345 bp

>T123113 NZ_CP038662:4190827-4191171 [Serratia nematodiphila]
ATGTGGCAAGTGGTGACCGTCGAACGGTTTGACGACTGGTTTTTAGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTACAAATTGCAGACGTTCGGCCCGCAATTGGCGCGGCCGCATGCCGATACGCTGCATTTTTCCGACGCGGTCC
GACAGTTGAAGGAGCTGCGCATTCAACATCGCGGGTGCCCTTTCAGGGTGTTTTTCGCCTTTGATCCACAGCGGCAAGCG
GTATTGCTGTGCGGCGGCGATAAAACCGGCGACAAACGCTTTTACCAGCGCATGTTGCCCATCGCCGCCATGGAGTTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11080.97 Da
• Isoelectric Point: 10.1998

>AT123113 WP_004931828.1 NZ_CP038662:4191177-4191479 [Serratia nematodiphila]
MAKPLSQLIDKLDPAVVAAARQKADQEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLDVQLPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT123113 NZ_CP038662:4191177-4191479 [Serratia nematodiphila]
ATGGCCAAACCTTTATCCCAACTGATCGATAAACTCGATCCCGCCGTCGTTGCCGCCGCCAGGCAAAAAGCGGACCAGGA
GATTTTCGAGCTGCGGCTGGCGATGCTGAGGGAAGAGTTGGCGGTTTCTCAGGTTGAATTGGCGAAGCGGCTCGGCATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATCAAGCTGTCATCGCTCAAACGCTATATTGAGGCGATG
GGCGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAGCACCGCCGCATGACGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A086GAN0