Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 457215..457827 Replicon chromosome
Accession NZ_CP038634
Organism Cupriavidus oxalaticus strain X32

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag E0W60_RS01985 Protein ID WP_135702850.1
Coordinates 457215..457487 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E0W60_RS01990 Protein ID WP_133094486.1
Coordinates 457498..457827 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0W60_RS01985 457215..457487 + 273 WP_135702850.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E0W60_RS01990 457498..457827 + 330 WP_133094486.1 HigA family addiction module antidote protein Antitoxin
E0W60_RS01995 457876..459234 - 1359 WP_135702851.1 serine hydrolase -
E0W60_RS02000 459285..459767 - 483 WP_133094484.1 MarR family transcriptional regulator -
E0W60_RS02005 459968..460564 - 597 WP_133094483.1 GTP cyclohydrolase II -
E0W60_RS02010 460768..461238 - 471 WP_133094482.1 Lrp/AsnC family transcriptional regulator -
E0W60_RS02015 461391..461753 + 363 WP_133094481.1 AsnC family transcriptional regulator -
E0W60_RS02020 461763..462680 - 918 WP_135702852.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(24-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-66)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10247.69 Da        Isoelectric Point: 7.3228

>T123083 WP_135702850.1 NZ_CP038634:457215-457487 [Cupriavidus oxalaticus]
MIQSFRCSDTAALFDGHYVARFASIEFVAQRKLEMLDDAATLGALRFPPGNRLEKLGGDRRHSLRINGQWRVCFIWTHDG
PAAVEIIDYH

Download         Length: 273 bp

>T123083 NZ_CP038634:457215-457487 [Cupriavidus oxalaticus]
ATGATCCAATCCTTCAGATGTTCCGACACCGCTGCACTGTTCGACGGCCATTACGTCGCCCGCTTTGCGAGCATCGAGTT
CGTTGCCCAGCGCAAGCTGGAGATGCTTGATGACGCCGCCACGTTGGGTGCGCTGCGCTTTCCGCCGGGCAACCGCCTGG
AAAAACTAGGCGGTGATCGCCGCCACAGCCTCCGCATCAACGGCCAGTGGAGAGTCTGCTTCATCTGGACCCATGACGGC
CCCGCGGCTGTCGAGATCATCGACTACCACTGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12302.12 Da        Isoelectric Point: 7.0167

>AT123083 WP_133094486.1 NZ_CP038634:457498-457827 [Cupriavidus oxalaticus]
MARFENRMRPIHPGEILREEYLVPLGMSANALALALRVTPARINEIVREQRGVTPDTALRLARYFGGDARSWMNMQVTYD
LKVAQRDLGERIAAEVVPREGAGQEIPEN

Download         Length: 330 bp

>AT123083 NZ_CP038634:457498-457827 [Cupriavidus oxalaticus]
ATGGCACGCTTTGAAAACCGCATGCGCCCGATCCATCCGGGCGAAATCCTGCGCGAGGAATACCTGGTGCCGCTGGGCAT
GAGCGCCAATGCCCTGGCGCTGGCGTTGCGCGTGACGCCGGCCCGCATCAACGAGATCGTGCGCGAGCAGCGCGGCGTCA
CGCCAGACACCGCGTTGCGCCTGGCGCGCTATTTCGGTGGCGATGCGCGTTCGTGGATGAATATGCAGGTGACCTACGAC
CTGAAGGTCGCGCAGCGGGATCTGGGCGAGCGCATCGCGGCGGAAGTGGTGCCGCGCGAGGGCGCCGGCCAGGAAATTCC
GGAAAACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References