Detailed information of TA system TA123060

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 364288..364796
• Replicon: chromosome
• Accession: NZ_CP038612
• Organism: Staphylococcus aureus strain O268

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: SaO268_RS01780
• Protein ID: WP_000448764.1
• Coordinates: 364494..364796 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	364288..364398 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO268_RS01740	359382..359672	+	291	WP_000179860.1	hypothetical protein	-
SaO268_RS01745	359688..361598	+	1911	WP_000429563.1	hypothetical protein	-
SaO268_RS01750	361598..363064	+	1467	WP_000067157.1	BppU family phage baseplate upper protein	-
SaO268_RS01755	363064..363453	+	390	WP_001166604.1	DUF2977 domain-containing protein	-
SaO268_RS01760	363446..363610	+	165	WP_000916020.1	XkdX family protein	-
SaO268_RS01765	363656..363955	+	300	WP_000466773.1	DUF2951 family protein	-
SaO268_RS14885	364107..364296	+	190	Protein_336	putative holin-like toxin	-
-	364288..364398	-	111	NuclAT_0	-	Antitoxin
-	364288..364398	-	111	NuclAT_0	-	Antitoxin
-	364288..364398	-	111	NuclAT_0	-	Antitoxin
-	364288..364398	-	111	NuclAT_0	-	Antitoxin
SaO268_RS01775	364343..364443	-	101	Protein_337	hypothetical protein	-
SaO268_RS01780	364494..364796	+	303	WP_000448764.1	phage holin	Toxin
SaO268_RS01785	364808..366262	+	1455	WP_000930278.1	N-acetylmuramoyl-L-alanine amidase	-
SaO268_RS01795	367776..368273	+	498	WP_001803960.1	hypothetical protein	-
SaO268_RS01805	368833..369660	-	828	WP_000136028.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	321211..366262	45051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11230.99 Da
• Isoelectric Point: 9.6587

>T123060 WP_000448764.1 NZ_CP038612:364494-364796 [Staphylococcus aureus]
METKVITRYIVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTAYKDNPTSQEGRWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T123060 NZ_CP038612:364494-364796 [Staphylococcus aureus]
ATGGAAACAAAAGTAATAACAAGATATATTGTATTAATTTTAGCGTTAGTCAATCAGTTTTTAGCGAACAAAGGTATAAG
CCCGATACCAGTAGATGAAGAGAGTATTTCATCGATTATCTTAACAGTCATTGCTTTATACACCGCTTATAAAGACAATC
CAACATCACAAGAAGGACGCTGGGCGAATCAAAAACTAAAGAAATATAAAGCTGAAAATAAGTATAGAAAAGCAACAGGA
CAAGCGCCAATTAAAGAAGTAATGACACCTACAAATATGAATGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 111 bp

>AT123060 NZ_CP038612:c364398-364288 [Staphylococcus aureus]
AAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGACCTCTGTAGTTAAATGAATTTATATAATCCTCTAACCATCG
TACTCGTCAAAGTTAGCGATGGTTATTTTTT

Structures

Antitoxin