Detailed information of TA system TA123056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5126216..5126818
• Replicon: chromosome
• Accession: NZ_CP038505
• Organism: Escherichia coli strain 28Eco12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: DC440_RS25355
• Protein ID: WP_000897305.1
• Coordinates: 5126507..5126818 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DC440_RS25350
• Protein ID: WP_000356395.1
• Coordinates: 5126216..5126506 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DC440_RS25310	5121840..5122742	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DC440_RS25315	5122739..5123374	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DC440_RS25320	5123371..5124300	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
DC440_RS25325	5124482..5124724	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
DC440_RS25330	5124943..5125161	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
DC440_RS25340	5125580..5125858	-	279	WP_001315112.1	hypothetical protein	-
DC440_RS25345	5125910..5126131	-	222	WP_001550354.1	hypothetical protein	-
DC440_RS25350	5126216..5126506	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
DC440_RS25355	5126507..5126818	-	312	WP_000897305.1	hypothetical protein	Toxin
DC440_RS25360	5127047..5127955	+	909	WP_001550353.1	alpha/beta hydrolase	-
DC440_RS25365	5128123..5129937	-	1815	WP_001550352.1	hypothetical protein	-
DC440_RS25370	5130353..5131294	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DC440_RS25375	5131339..5131776	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T123056 WP_000897305.1 NZ_CP038505:c5126818-5126507 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T123056 NZ_CP038505:c5126818-5126507 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT123056 WP_000356395.1 NZ_CP038505:c5126506-5126216 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT123056 NZ_CP038505:c5126506-5126216 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7