Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1990986..1991488 Replicon chromosome
Accession NZ_CP038505
Organism Escherichia coli strain 28Eco12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DC440_RS09645 Protein ID WP_000767829.1
Coordinates 1991234..1991488 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DC440_RS09640 Protein ID WP_001259255.1
Coordinates 1990986..1991237 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DC440_RS09615 1986164..1987231 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DC440_RS09620 1987231..1988301 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
DC440_RS09625 1988298..1989602 - 1305 WP_021523144.1 histidinol dehydrogenase -
DC440_RS09630 1989608..1990507 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
DC440_RS09635 1990653..1990703 - 51 WP_001364200.1 his operon leader peptide -
DC440_RS09640 1990986..1991237 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DC440_RS09645 1991234..1991488 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DC440_RS09650 1991571..1992395 + 825 WP_000754758.1 SDR family oxidoreductase -
DC440_RS09655 1992441..1993370 + 930 WP_023154044.1 LysR family transcriptional regulator -
DC440_RS09660 1993585..1993647 + 63 WP_010723108.1 membrane protein YoeI -
DC440_RS09665 1993637..1994995 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DC440_RS09670 1995174..1996232 + 1059 WP_000492324.1 transport protein YeeE -
DC440_RS09675 1996246..1996473 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T123033 WP_000767829.1 NZ_CP038505:1991234-1991488 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T123033 NZ_CP038505:1991234-1991488 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT123033 WP_001259255.1 NZ_CP038505:1990986-1991237 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT123033 NZ_CP038505:1990986-1991237 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References