Detailed information of TA system TA123022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14234..14885
• Replicon: plasmid unnamed2
• Accession: NZ_CP038502
• Organism: Acinetobacter baumannii strain CIAT758

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: E4664_RS20290
• Protein ID: WP_000269904.1
• Coordinates: 14234..14590 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4664_RS20295
• Protein ID: WP_001129974.1
• Coordinates: 14583..14885 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4664_RS20260	10968..11153	+	186	WP_000755725.1	hypothetical protein	-
E4664_RS20265	11156..11803	+	648	WP_000464611.1	Fic family protein	-
E4664_RS20270	11910..12239	-	330	WP_031974808.1	DUF4236 domain-containing protein	-
E4664_RS20275	12497..13201	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
E4664_RS20285	13390..13706	+	317	Protein_9	hypothetical protein	-
E4664_RS20290	14234..14590	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4664_RS20295	14583..14885	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
E4664_RS20300	14878..15087	+	210	WP_000069475.1	hypothetical protein	-
E4664_RS20305	15262..15780	+	519	WP_000447195.1	sel1 repeat family protein	-
E4664_RS20310	15819..16331	+	513	WP_001089574.1	SMI1/KNR4 family protein	-
E4664_RS20315	16570..17273	+	704	Protein_15	IS6-like element IS26 family transposase	-
E4664_RS20320	17325..18392	+	1068	Protein_16	transposase	-
E4664_RS20325	18717..19643	+	927	WP_032495440.1	class A extended-spectrum beta-lactamase PER-7	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaPER-7 / sul1 / aph(3')-VIa	-	1..47417	47417
	-	inside	IScluster/Tn	blaPER-7 / sul1 / aph(3')-VIa	-	12497..30825	18328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T123022 WP_000269904.1 NZ_CP038502:14234-14590 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T123022 NZ_CP038502:14234-14590 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT123022 WP_001129974.1 NZ_CP038502:14583-14885 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT123022 NZ_CP038502:14583-14885 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1