Detailed information of TA system TA123019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3402..3987
• Replicon: plasmid unnamed1
• Accession: NZ_CP038501
• Organism: Acinetobacter baumannii strain CIAT758

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: E4664_RS19820
• Protein ID: WP_000897307.1
• Coordinates: 3667..3987 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4664_RS19815
• Protein ID: WP_000369781.1
• Coordinates: 3402..3674 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4664_RS19785	460..729	+	270	WP_039048204.1	hypothetical protein	-
E4664_RS19790	776..916	+	141	Protein_2	transposase domain-containing protein	-
E4664_RS19795	1059..1379	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4664_RS19800	1372..1644	+	273	WP_094069338.1	helix-turn-helix domain-containing protein	-
E4664_RS19805	2129..2899	-	771	WP_094069337.1	hypothetical protein	-
E4664_RS19810	2958..3296	+	339	WP_032073153.1	hypothetical protein	-
E4664_RS19815	3402..3674	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
E4664_RS19820	3667..3987	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4664_RS19825	4127..4546	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
E4664_RS19830	4626..4907	-	282	WP_000985609.1	putative addiction module antidote protein	-
E4664_RS19835	4908..5210	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4664_RS19840	5359..5893	+	535	Protein_12	hypothetical protein	-
E4664_RS19845	5982..6296	-	315	WP_004728120.1	BrnA antitoxin family protein	-
E4664_RS19850	6283..6570	-	288	WP_000438825.1	BrnT family toxin	-
E4664_RS19855	6866..7507	-	642	WP_167850447.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
E4664_RS19860	7573..8760	+	1188	WP_087551687.1	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / aac(3)-IId / msr(E) / mph(E) / blaOXA-58	-	1..78125	78125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T123019 WP_000897307.1 NZ_CP038501:c3987-3667 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T123019 NZ_CP038501:c3987-3667 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT123019 WP_000369781.1 NZ_CP038501:c3674-3402 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT123019 NZ_CP038501:c3674-3402 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3