Detailed information of TA system TA122981

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ldrD-agrB/Ldr(toxin)
• Location: 5224060..5224281
• Replicon: chromosome
• Accession: NZ_CP038496
• Organism: Escherichia coli O157:H7 strain TT12A

Toxin (Protein)

• Gene name: ldrD
• Uniprot ID: S1NWX0
• Locus tag: E5F08_RS25860
• Protein ID: WP_001295224.1
• Coordinates: 5224060..5224167 (-)
• Length: 36 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	5224216..5224281 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5F08_RS25835	5219313..5220065	-	753	Protein_5035	cellulose biosynthesis protein BcsQ	-
E5F08_RS25840	5220077..5220265	-	189	WP_001063316.1	YhjR family protein	-
E5F08_RS25845	5220538..5222109	+	1572	WP_001204920.1	cellulose biosynthesis protein BcsE	-
E5F08_RS25850	5222106..5222297	+	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
E5F08_RS25855	5222294..5223973	+	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
E5F08_RS25860	5224060..5224167	-	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	Toxin
-	5224216..5224281	+	66	NuclAT_17	-	Antitoxin
-	5224216..5224281	+	66	NuclAT_17	-	Antitoxin
-	5224216..5224281	+	66	NuclAT_17	-	Antitoxin
-	5224216..5224281	+	66	NuclAT_17	-	Antitoxin
-	5224216..5224281	+	66	NuclAT_22	-	Antitoxin
-	5224216..5224281	+	66	NuclAT_22	-	Antitoxin
-	5224216..5224281	+	66	NuclAT_22	-	Antitoxin
-	5224216..5224281	+	66	NuclAT_22	-	Antitoxin
E5F08_RS25865	5224643..5225914	+	1272	WP_001301684.1	amino acid permease	-
E5F08_RS25870	5225944..5226948	-	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
E5F08_RS25875	5226945..5227928	-	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	-
E5F08_RS25880	5227939..5228841	-	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3882.70 Da
• Isoelectric Point: 9.0157

>T122981 WP_001295224.1 NZ_CP038496:c5224167-5224060 [Escherichia coli O157:H7]
MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK

Nucleotide

• Length: 108 bp

>T122981 NZ_CP038496:c5224167-5224060 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT122981 NZ_CP038496:5224216-5224281 [Escherichia coli O157:H7]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4P7TSJ7

Antitoxin