Detailed information of TA system TA122958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1030505..1031199
• Replicon: chromosome
• Accession: NZ_CP038496
• Organism: Escherichia coli O157:H7 strain TT12A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E5F08_RS04650
• Protein ID: WP_001263495.1
• Coordinates: 1030801..1031199 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E5F08_RS04645
• Protein ID: WP_000554757.1
• Coordinates: 1030505..1030798 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5F08_RS04625	1026216..1026713	+	498	Protein_883	REP-associated tyrosine transposase RayT	-
E5F08_RS04630	1026858..1028570	-	1713	Protein_884	flagellar biosynthesis protein FlhA	-
E5F08_RS04635	1028542..1029327	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E5F08_RS04640	1029398..1030453	+	1056	WP_001226188.1	DNA polymerase IV	-
E5F08_RS04645	1030505..1030798	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E5F08_RS04650	1030801..1031199	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E5F08_RS04655	1031209..1031661	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E5F08_RS04660	1031980..1032183	+	204	Protein_890	RNA ligase RtcB family protein	-
E5F08_RS04665	1032182..1032703	+	522	Protein_891	peptide chain release factor H	-
E5F08_RS04670	1032760..1034217	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E5F08_RS04675	1034478..1034936	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1035532..1035612	+	81	NuclAT_12	-	-
-	1035532..1035612	+	81	NuclAT_12	-	-
-	1035532..1035612	+	81	NuclAT_12	-	-
-	1035532..1035612	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T122958 WP_001263495.1 NZ_CP038496:1030801-1031199 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T122958 NZ_CP038496:1030801-1031199 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT122958 WP_000554757.1 NZ_CP038496:1030505-1030798 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT122958 NZ_CP038496:1030505-1030798 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6