Detailed information of TA system TA122921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2894539..2895053
• Replicon: chromosome
• Accession: NZ_CP038492
• Organism: Boseongicola sp. CCM32

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E2K80_RS14080
• Protein ID: WP_168193264.1
• Coordinates: 2894847..2895053 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E2K80_RS14075
• Protein ID: WP_135375574.1
• Coordinates: 2894539..2894823 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2K80_RS14060	2889557..2892970	+	3414	WP_135375571.1	efflux RND transporter permease subunit	-
E2K80_RS14065	2892974..2893798	-	825	WP_168193200.1	hypothetical protein	-
E2K80_RS14070	2893915..2894505	+	591	WP_135375573.1	YqgE/AlgH family protein	-
E2K80_RS14075	2894539..2894823	-	285	WP_135375574.1	HigA family addiction module antidote protein	Antitoxin
E2K80_RS14080	2894847..2895053	-	207	WP_168193264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2K80_RS14085	2895302..2896957	-	1656	WP_135375575.1	energy-dependent translational throttle protein EttA	-
E2K80_RS14090	2897249..2897782	+	534	WP_135375576.1	adenylate kinase	-
E2K80_RS14095	2898177..2898392	+	216	WP_135375577.1	hypothetical protein	-
E2K80_RS14100	2898507..2899109	+	603	WP_135375578.1	nicotinate-nucleotide adenylyltransferase	-
E2K80_RS14105	2899143..2899997	-	855	WP_135375579.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-68)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-69)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7747.80 Da
• Isoelectric Point: 7.1648

>T122921 WP_168193264.1 NZ_CP038492:c2895053-2894847 [Boseongicola sp. CCM32]
IFEAATRKITMLDAADTLNDLRVPPANRLEALKGNRAGQHSIRINDQWRICFIWTRAGPADVELTDYH

Nucleotide

• Length: 207 bp

>T122921 NZ_CP038492:c2895053-2894847 [Boseongicola sp. CCM32]
ATTTTCGAAGCCGCAACGCGGAAAATAACAATGCTTGATGCTGCGGACACCCTGAACGACTTGCGCGTCCCGCCCGCCAA
CCGGCTTGAAGCGTTAAAAGGCAATCGCGCGGGGCAGCATTCGATCCGCATCAATGACCAGTGGCGGATCTGCTTTATCT
GGACCCGGGCCGGTCCTGCTGATGTTGAACTGACAGACTACCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10713.51 Da
• Isoelectric Point: 10.7496

>AT122921 WP_135375574.1 NZ_CP038492:c2894823-2894539 [Boseongicola sp. CCM32]
MHATLKNPVHPGAILKHEFLLPLCLSEGQLAKRLGVPRTRIERLTKGTTSMTPDTAFRLARFFGTTPQFWMNMQINHDIT
HTQVDVSHIEPLRV

Nucleotide

• Length: 285 bp

>AT122921 NZ_CP038492:c2894823-2894539 [Boseongicola sp. CCM32]
TTGCATGCGACATTGAAAAATCCGGTGCATCCCGGTGCAATTCTCAAACATGAATTCCTGCTGCCGCTCTGCCTGAGCGA
GGGTCAGCTTGCCAAACGCCTCGGCGTACCACGCACCCGTATCGAACGGTTGACGAAGGGCACCACCTCAATGACCCCGG
ACACCGCCTTTCGGCTGGCGCGTTTCTTCGGAACCACACCGCAATTCTGGATGAATATGCAGATCAATCATGACATCACG
CATACGCAGGTGGATGTCAGCCATATCGAGCCGCTGCGCGTCTAA