Detailed information of TA system TA122857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 355041..355559
• Replicon: chromosome
• Accession: NZ_CP038461
• Organism: Staphylococcus aureus strain O217

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: SaO217_RS01655
• Protein ID: WP_000448764.1
• Coordinates: 355257..355559 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	355041..355177 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO217_RS01615	350135..350425	+	291	WP_000179860.1	hypothetical protein	-
SaO217_RS01620	350441..352351	+	1911	WP_000429563.1	hypothetical protein	-
SaO217_RS01625	352351..353817	+	1467	WP_000067157.1	BppU family phage baseplate upper protein	-
SaO217_RS01630	353817..354206	+	390	WP_001166604.1	DUF2977 domain-containing protein	-
SaO217_RS01635	354199..354363	+	165	WP_000916020.1	XkdX family protein	-
SaO217_RS01640	354409..354708	+	300	WP_000466773.1	DUF2951 family protein	-
SaO217_RS01645	354860..355049	+	190	Protein_326	putative holin-like toxin	-
-	355041..355177	-	137	NuclAT_0	-	Antitoxin
-	355041..355177	-	137	NuclAT_0	-	Antitoxin
-	355041..355177	-	137	NuclAT_0	-	Antitoxin
-	355041..355177	-	137	NuclAT_0	-	Antitoxin
SaO217_RS01650	355096..355206	-	111	WP_070003492.1	hypothetical protein	-
SaO217_RS01655	355257..355559	+	303	WP_000448764.1	phage holin	Toxin
SaO217_RS01660	355571..357025	+	1455	WP_000930278.1	N-acetylmuramoyl-L-alanine amidase	-
SaO217_RS01665	358539..359036	+	498	WP_001803960.1	hypothetical protein	-
SaO217_RS01670	359596..360423	-	828	WP_000136022.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	geh	304276..357025	52749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11230.99 Da
• Isoelectric Point: 9.6587

>T122857 WP_000448764.1 NZ_CP038461:355257-355559 [Staphylococcus aureus]
METKVITRYIVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTAYKDNPTSQEGRWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T122857 NZ_CP038461:355257-355559 [Staphylococcus aureus]
ATGGAAACAAAAGTAATAACAAGATATATTGTATTAATTTTAGCGTTAGTCAATCAGTTTTTAGCGAACAAAGGTATAAG
CCCGATACCAGTAGATGAAGAGAGTATTTCATCGATTATCTTAACAGTCATTGCTTTATACACCGCTTATAAAGACAATC
CAACATCACAAGAAGGACGCTGGGCGAATCAAAAACTAAAGAAATATAAAGCTGAAAATAAGTATAGAAAAGCAACAGGA
CAAGCGCCAATTAAAGAAGTAATGACACCTACAAATATGAATGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 137 bp

>AT122857 NZ_CP038461:c355177-355041 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGACCTCTGTAGTTAAAT
GAATTTATATAATCCTCTAACCATCGTACTCGTCAAAGTTAGCGATGGTTATTTTTT

Structures

Antitoxin