Detailed information of TA system TA122835

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85682..86108
• Replicon: plasmid pEC129_2
• Accession: NZ_CP038455
• Organism: Escherichia coli strain EC-129

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: E4Z89_RS25090
• Protein ID: WP_034169443.1
• Coordinates: 85682..85840 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	85884..86108 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4Z89_RS25045	80732..80959	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
E4Z89_RS25050	81053..81739	-	687	WP_001825184.1	PAS domain-containing protein	-
E4Z89_RS25055	81930..82313	-	384	WP_000124981.1	relaxosome protein TraM	-
E4Z89_RS25060	82590..83237	+	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
E4Z89_RS25065	83534..84355	-	822	WP_001234469.1	DUF945 domain-containing protein	-
E4Z89_RS25070	84473..84760	-	288	WP_000107535.1	hypothetical protein	-
E4Z89_RS25075	84785..84991	-	207	WP_000547971.1	hypothetical protein	-
E4Z89_RS25090	85682..85840	-	159	WP_034169443.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	85884..86108	-	225	NuclAT_0	-	Antitoxin
-	85884..86108	-	225	NuclAT_0	-	Antitoxin
-	85884..86108	-	225	NuclAT_0	-	Antitoxin
-	85884..86108	-	225	NuclAT_0	-	Antitoxin
E4Z89_RS26650	85920..86108	+	189	WP_001299721.1	hypothetical protein	-
E4Z89_RS25095	86120..86839	-	720	WP_050858785.1	plasmid SOS inhibition protein A	-
E4Z89_RS25100	86836..87270	-	435	WP_032217500.1	conjugation system SOS inhibitor PsiB	-
E4Z89_RS25105	87325..89283	-	1959	WP_072652246.1	ParB/RepB/Spo0J family partition protein	-
E4Z89_RS25110	89348..89581	-	234	WP_000006030.1	DUF905 family protein	-
E4Z89_RS25115	89643..90182	-	540	WP_009426937.1	single-stranded DNA-binding protein	-
E4Z89_RS25120	90208..90414	-	207	WP_000547971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / dfrA27 / aadA16 / sul1 / rmtB / blaTEM-1B / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A)	-	1..117599	117599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T122835 WP_034169443.1 NZ_CP038455:c85840-85682 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCELRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T122835 NZ_CP038455:c85840-85682 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGCTTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT122835 NZ_CP038455:c86108-85884 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin