Detailed information of TA system TA122829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3787677..3788371
• Replicon: chromosome
• Accession: NZ_CP038453
• Organism: Escherichia coli strain EC-129

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: E4Z89_RS18485
• Protein ID: WP_001263489.1
• Coordinates: 3787677..3788075 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: E4Z89_RS18490
• Protein ID: WP_000554758.1
• Coordinates: 3788078..3788371 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3783265..3783345	-	81	NuclAT_12	-	-
-	3783265..3783345	-	81	NuclAT_12	-	-
-	3783265..3783345	-	81	NuclAT_12	-	-
-	3783265..3783345	-	81	NuclAT_12	-	-
E4Z89_RS18460	3783941..3784399	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E4Z89_RS18465	3784660..3786117	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
E4Z89_RS18470	3786174..3786731	-	558	WP_001352051.1	peptide chain release factor H	-
E4Z89_RS18475	3786643..3786909	-	267	WP_001295202.1	hypothetical protein	-
E4Z89_RS18480	3787215..3787667	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
E4Z89_RS18485	3787677..3788075	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E4Z89_RS18490	3788078..3788371	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E4Z89_RS18495	3788423..3789478	-	1056	WP_001226164.1	DNA polymerase IV	-
E4Z89_RS18500	3789549..3790334	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
E4Z89_RS18505	3790279..3792018	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
E4Z89_RS18510	3792242..3792739	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T122829 WP_001263489.1 NZ_CP038453:c3788075-3787677 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T122829 NZ_CP038453:c3788075-3787677 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT122829 WP_000554758.1 NZ_CP038453:c3788371-3788078 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT122829 NZ_CP038453:c3788371-3788078 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3