Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3787677..3788371 Replicon chromosome
Accession NZ_CP038453
Organism Escherichia coli strain EC-129

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag E4Z89_RS18485 Protein ID WP_001263489.1
Coordinates 3787677..3788075 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag E4Z89_RS18490 Protein ID WP_000554758.1
Coordinates 3788078..3788371 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3783265..3783345 - 81 NuclAT_12 - -
- 3783265..3783345 - 81 NuclAT_12 - -
- 3783265..3783345 - 81 NuclAT_12 - -
- 3783265..3783345 - 81 NuclAT_12 - -
E4Z89_RS18460 3783941..3784399 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
E4Z89_RS18465 3784660..3786117 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
E4Z89_RS18470 3786174..3786731 - 558 WP_001352051.1 peptide chain release factor H -
E4Z89_RS18475 3786643..3786909 - 267 WP_001295202.1 hypothetical protein -
E4Z89_RS18480 3787215..3787667 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
E4Z89_RS18485 3787677..3788075 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E4Z89_RS18490 3788078..3788371 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E4Z89_RS18495 3788423..3789478 - 1056 WP_001226164.1 DNA polymerase IV -
E4Z89_RS18500 3789549..3790334 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
E4Z89_RS18505 3790279..3792018 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
E4Z89_RS18510 3792242..3792739 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T122829 WP_001263489.1 NZ_CP038453:c3788075-3787677 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T122829 NZ_CP038453:c3788075-3787677 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT122829 WP_000554758.1 NZ_CP038453:c3788371-3788078 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT122829 NZ_CP038453:c3788371-3788078 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References