Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1856439..1856941 Replicon chromosome
Accession NZ_CP038453
Organism Escherichia coli strain EC-129

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag E4Z89_RS08865 Protein ID WP_000767831.1
Coordinates 1856687..1856941 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E4Z89_RS08860 Protein ID WP_001259255.1
Coordinates 1856439..1856690 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z89_RS08835 1851616..1852683 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E4Z89_RS08840 1852683..1853753 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
E4Z89_RS08845 1853750..1855054 - 1305 WP_001446927.1 histidinol dehydrogenase -
E4Z89_RS08850 1855060..1855959 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E4Z89_RS08855 1856105..1856155 - 51 WP_001364200.1 his operon leader peptide -
E4Z89_RS08860 1856439..1856690 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E4Z89_RS08865 1856687..1856941 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E4Z89_RS08870 1857024..1857848 + 825 WP_000754737.1 SDR family oxidoreductase -
E4Z89_RS08875 1857894..1858823 + 930 WP_024195433.1 LysR family transcriptional regulator -
E4Z89_RS08880 1859038..1859100 + 63 WP_010723108.1 membrane protein YoeI -
E4Z89_RS08885 1859090..1860448 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E4Z89_RS08890 1860627..1861685 + 1059 WP_000492321.1 transport protein YeeE -
E4Z89_RS08895 1861699..1861926 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - galF / cpsACP / wzi / wza / gnd / wbtL / ugd / fyuA 1681644..1914813 233169


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T122815 WP_000767831.1 NZ_CP038453:1856687-1856941 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T122815 NZ_CP038453:1856687-1856941 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT122815 WP_001259255.1 NZ_CP038453:1856439-1856690 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT122815 NZ_CP038453:1856439-1856690 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References