Detailed information of TA system TA122813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1110888..1111615
• Replicon: chromosome
• Accession: NZ_CP038453
• Organism: Escherichia coli strain EC-129

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: E4Z89_RS05335
• Protein ID: WP_000547564.1
• Coordinates: 1110888..1111199 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4Z89_RS05340
• Protein ID: WP_000126294.1
• Coordinates: 1111196..1111615 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4Z89_RS05305	1106033..1107742	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
E4Z89_RS05310	1107752..1108294	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
E4Z89_RS05315	1108294..1109061	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
E4Z89_RS05320	1109058..1109468	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
E4Z89_RS05325	1109461..1109928	+	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
E4Z89_RS05330	1109971..1110714	+	744	WP_097754117.1	hypothetical protein	-
E4Z89_RS05335	1110888..1111199	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
E4Z89_RS05340	1111196..1111615	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
E4Z89_RS05345	1111729..1113153	-	1425	WP_097754125.1	6-phospho-beta-glucosidase AscB	-
E4Z89_RS05350	1113162..1114619	-	1458	WP_001107862.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
E4Z89_RS05355	1114879..1115889	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
E4Z89_RS05360	1116038..1116565	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T122813 WP_000547564.1 NZ_CP038453:1110888-1111199 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T122813 NZ_CP038453:1110888-1111199 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT122813 WP_000126294.1 NZ_CP038453:1111196-1111615 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT122813 NZ_CP038453:1111196-1111615 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5