Detailed information of TA system TA122808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 46414..46915
• Replicon: plasmid pAeme5
• Accession: NZ_CP038449
• Organism: Aeromonas media strain R50-22

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: E4188_RS22150
• Protein ID: WP_171270028.1
• Coordinates: 46646..46915 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: E4188_RS22145
• Protein ID: WP_171270029.1
• Coordinates: 46414..46659 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4188_RS22140	44279..45289	-	1011	WP_171270030.1	tetratricopeptide repeat protein	-
E4188_RS22145	46414..46659	+	246	WP_171270029.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E4188_RS22150	46646..46915	+	270	WP_171270028.1	Txe/YoeB family addiction module toxin	Toxin
E4188_RS22155	47070..47480	-	411	WP_171270027.1	hypothetical protein	-
E4188_RS22160	49930..50187	+	258	WP_171270026.1	hypothetical protein	-
E4188_RS22165	50789..51208	+	420	WP_171270025.1	hypothetical protein	-
E4188_RS22170	51245..51814	+	570	WP_171270024.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..198927	198927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (3-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10272.62 Da
• Isoelectric Point: 10.0996

>T122808 WP_171270028.1 NZ_CP038449:46646-46915 [Aeromonas media]
MWTINFTKNGADDFAHWKATDSTKLERIRALLNAIESDPFSGIGKPERLRHHKNPALYSRRIDQQHRLVYSVSGTTVTVI
ACRYHYGDK

Nucleotide

• Length: 270 bp

>T122808 NZ_CP038449:46646-46915 [Aeromonas media]
ATGTGGACTATTAATTTCACAAAGAACGGCGCTGATGACTTTGCTCATTGGAAGGCAACTGACTCGACAAAGCTGGAGCG
GATACGGGCCCTGCTGAATGCCATAGAGAGTGACCCTTTCAGTGGGATCGGCAAACCTGAGCGACTGCGACATCACAAGA
ATCCGGCGTTGTACTCGCGGCGCATCGACCAACAGCACCGGCTTGTTTACAGCGTGTCCGGTACGACCGTCACGGTGATC
GCCTGCCGCTACCACTACGGTGATAAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9125.22 Da
• Isoelectric Point: 4.2404

>AT122808 WP_171270029.1 NZ_CP038449:46414-46659 [Aeromonas media]
MQSITFSDARKQFAATMNRVTDNAEPVRIMRRDAPDIVMIDASEYEAMAETIYLFSNPANAAHLNESLAQLERGEVVNVD
Y

Nucleotide

• Length: 246 bp

>AT122808 NZ_CP038449:46414-46659 [Aeromonas media]
ATGCAATCCATAACCTTTTCTGACGCTCGCAAGCAGTTTGCGGCCACGATGAACCGTGTGACTGATAACGCCGAGCCGGT
ACGCATTATGCGGCGCGATGCACCCGATATAGTCATGATCGACGCGAGCGAGTACGAAGCGATGGCAGAGACGATCTATC
TGTTCAGCAATCCTGCGAATGCCGCCCACCTCAACGAGTCCCTGGCACAACTGGAGCGCGGTGAAGTTGTCAATGTGGAC
TATTAA