Detailed information of TA system TA122790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 311786..312390
• Replicon: chromosome
• Accession: NZ_CP038438
• Organism: Pseudomonas fluorescens strain LBUM677

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3KJP2
• Locus tag: E4T63_RS01375
• Protein ID: WP_011331970.1
• Coordinates: 312076..312390 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4T63_RS01370
• Protein ID: WP_098966690.1
• Coordinates: 311786..312073 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T63_RS01345	306916..307377	-	462	WP_007964698.1	ATP-dependent zinc protease	-
E4T63_RS01350	307612..308019	+	408	WP_098966686.1	RNA-binding S4 domain-containing protein	-
E4T63_RS01355	308186..308989	-	804	WP_135294746.1	phosphatase PAP2 family protein	-
E4T63_RS01360	309095..309997	+	903	WP_098966688.1	Hsp33 family molecular chaperone HslO	-
E4T63_RS01365	310179..311720	+	1542	WP_027611209.1	phosphoenolpyruvate carboxykinase	-
E4T63_RS01370	311786..312073	-	288	WP_098966690.1	helix-turn-helix domain-containing protein	Antitoxin
E4T63_RS01375	312076..312390	-	315	WP_011331970.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4T63_RS01380	312502..312801	-	300	WP_098966691.1	hypothetical protein	-
E4T63_RS01385	313144..314286	-	1143	WP_135294747.1	hypothetical protein	-
E4T63_RS01390	314379..314654	-	276	WP_098966695.1	hypothetical protein	-
E4T63_RS01395	314957..315460	+	504	WP_135294748.1	polysaccharide deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.76 Da
• Isoelectric Point: 10.3534

>T122790 WP_011331970.1 NZ_CP038438:c312390-312076 [Pseudomonas fluorescens]
MIFIETRIFTRRVKELLDDDTYAAFQKQLVVSPSIGDVIEGTGGIRKTRIAAKGHGKRGGARVIYYHFVSASQIGLLMIY
PKNEQHDLSSDERKALKVLIEKWR

Nucleotide

• Length: 315 bp

>T122790 NZ_CP038438:c312390-312076 [Pseudomonas fluorescens]
ATGATTTTTATTGAGACTCGGATCTTTACCCGTCGAGTAAAAGAGCTGCTGGATGATGACACGTACGCCGCTTTTCAGAA
GCAACTGGTTGTAAGCCCTTCGATTGGCGATGTAATTGAAGGCACAGGCGGGATTCGCAAAACCCGAATTGCAGCGAAAG
GACACGGCAAGCGAGGCGGAGCACGTGTTATTTACTACCACTTCGTTTCGGCGTCACAAATCGGGTTATTGATGATCTAC
CCTAAAAATGAACAACACGATCTGTCCAGCGATGAGCGTAAAGCACTCAAGGTCTTGATCGAAAAATGGAGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10777.34 Da
• Isoelectric Point: 6.8814

>AT122790 WP_098966690.1 NZ_CP038438:c312073-311786 [Pseudomonas fluorescens]
MSKFFDELMESVQEMDEIVRGERTPSREFHVDALQVKEIRKATGLTQTKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAH

Nucleotide

• Length: 288 bp

>AT122790 NZ_CP038438:c312073-311786 [Pseudomonas fluorescens]
ATGAGCAAATTTTTTGATGAGCTGATGGAAAGTGTGCAAGAGATGGACGAGATCGTACGAGGCGAGCGCACACCTTCTCG
CGAATTCCACGTTGATGCATTGCAGGTCAAAGAAATTCGTAAAGCTACCGGTTTGACCCAGACCAAATTTGCGGCACTGA
TCGACGTCCAACTCGGCACTCTACGAAATTGGGAACAGGGCCGTCGCGAGCCAACCGGGCCGGCAAAAGCTTTGCTCAAG
GCCATTCATAACGACCCGGTTCATGTTTTGAATGCATTGGCTCATTGA

Structures

Toxin

• AlphaFold DB: Q3KJP2