Detailed information of TA system TA122784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 5463721..5464415
• Replicon: chromosome
• Accession: NZ_CP038428
• Organism: Escherichia coli O157:H7 strain 611

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E3149_RS26985
• Protein ID: WP_001263495.1
• Coordinates: 5464017..5464415 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E3149_RS26980
• Protein ID: WP_000554757.1
• Coordinates: 5463721..5464014 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3149_RS26960	5459432..5459929	+	498	Protein_5260	REP-associated tyrosine transposase RayT	-
E3149_RS26965	5460074..5461813	-	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E3149_RS26970	5461758..5462543	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E3149_RS26975	5462614..5463669	+	1056	WP_001226188.1	DNA polymerase IV	-
E3149_RS26980	5463721..5464014	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E3149_RS26985	5464017..5464415	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E3149_RS26990	5464425..5464877	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E3149_RS26995	5465184..5465450	+	267	WP_001303804.1	hypothetical protein	-
E3149_RS27000	5465362..5465919	+	558	WP_023438539.1	peptide chain release factor H	-
E3149_RS27005	5465976..5467433	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E3149_RS27010	5467694..5468152	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	5468748..5468828	+	81	NuclAT_12	-	-
-	5468748..5468828	+	81	NuclAT_12	-	-
-	5468748..5468828	+	81	NuclAT_12	-	-
-	5468748..5468828	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T122784 WP_001263495.1 NZ_CP038428:5464017-5464415 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T122784 NZ_CP038428:5464017-5464415 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT122784 WP_000554757.1 NZ_CP038428:5463721-5464014 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT122784 NZ_CP038428:5463721-5464014 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6