Detailed information of TA system TA122778

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ldrD-agrB/Ldr(toxin)
• Location: 4156262..4156483
• Replicon: chromosome
• Accession: NZ_CP038428
• Organism: Escherichia coli O157:H7 strain 611

Toxin (Protein)

• Gene name: ldrD
• Uniprot ID: S1NWX0
• Locus tag: E3149_RS21030
• Protein ID: WP_001295224.1
• Coordinates: 4156262..4156369 (-)
• Length: 36 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4156418..4156483 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3149_RS21005	4151515..4152267	-	753	Protein_4112	cellulose biosynthesis protein BcsQ	-
E3149_RS21010	4152279..4152467	-	189	WP_001063316.1	YhjR family protein	-
E3149_RS21015	4152740..4154311	+	1572	WP_001204920.1	cellulose biosynthesis protein BcsE	-
E3149_RS21020	4154308..4154499	+	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
E3149_RS21025	4154496..4156175	+	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
E3149_RS21030	4156262..4156369	-	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	Toxin
-	4156418..4156483	+	66	NuclAT_17	-	Antitoxin
-	4156418..4156483	+	66	NuclAT_17	-	Antitoxin
-	4156418..4156483	+	66	NuclAT_17	-	Antitoxin
-	4156418..4156483	+	66	NuclAT_17	-	Antitoxin
-	4156418..4156483	+	66	NuclAT_22	-	Antitoxin
-	4156418..4156483	+	66	NuclAT_22	-	Antitoxin
-	4156418..4156483	+	66	NuclAT_22	-	Antitoxin
-	4156418..4156483	+	66	NuclAT_22	-	Antitoxin
E3149_RS21035	4156845..4158116	+	1272	WP_001301684.1	amino acid permease	-
E3149_RS21040	4158146..4159150	-	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
E3149_RS21045	4159147..4160130	-	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	-
E3149_RS21050	4160141..4161043	-	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3882.70 Da
• Isoelectric Point: 9.0157

>T122778 WP_001295224.1 NZ_CP038428:c4156369-4156262 [Escherichia coli O157:H7]
MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK

Nucleotide

• Length: 108 bp

>T122778 NZ_CP038428:c4156369-4156262 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT122778 NZ_CP038428:4156418-4156483 [Escherichia coli O157:H7]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4P7TSJ7

Antitoxin