Detailed information of TA system TA122756

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1664943..1665314
• Replicon: chromosome
• Accession: NZ_CP038428
• Organism: Escherichia coli O157:H7 strain 611

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: E3149_RS08565
• Protein ID: WP_001443846.1
• Coordinates: 1665165..1665314 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1664943..1665121 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3149_RS08535	1660695..1660865	+	171	WP_001625136.1	hypothetical protein	-
E3149_RS08540	1660898..1662271	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
E3149_RS08545	1662400..1663335	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E3149_RS08550	1663387..1664622	-	1236	WP_000040839.1	site-specific integrase	-
E3149_RS08555	1664624..1664839	-	216	WP_000079604.1	excisionase XisR	-
E3149_RS08560	1664939..1665127	-	189	WP_001302840.1	DUF1187 family protein	-
-	1664943..1665121	+	179	NuclAT_0	-	Antitoxin
-	1664943..1665121	+	179	NuclAT_0	-	Antitoxin
-	1664943..1665121	+	179	NuclAT_0	-	Antitoxin
-	1664943..1665121	+	179	NuclAT_0	-	Antitoxin
E3149_RS08565	1665165..1665314	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E3149_RS08570	1665370..1666179	-	810	WP_000166313.1	recombination protein RecT	-
E3149_RS08575	1666172..1668772	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
E3149_RS08580	1668874..1669149	-	276	WP_001344816.1	hypothetical protein	-
E3149_RS08585	1669224..1669394	-	171	WP_001352098.1	conserved protein, Rac prophage	-
E3149_RS08590	1669394..1669615	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	1656128..1763507	107379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T122756 WP_001443846.1 NZ_CP038428:c1665314-1665165 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T122756 NZ_CP038428:c1665314-1665165 [Escherichia coli O157:H7]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT122756 NZ_CP038428:1664943-1665121 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin