Detailed information of TA system TA122707

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3543690..3543915
• Replicon: chromosome
• Accession: NZ_CP038423
• Organism: Escherichia coli O157:H7 strain 7409

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3151_RS18205
• Protein ID: WP_000813263.1
• Coordinates: 3543690..3543845 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3543857..3543915 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3151_RS18170	3539144..3539857	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E3151_RS18175	3539995..3540191	-	197	Protein_3558	TrmB family transcriptional regulator	-
E3151_RS18180	3540478..3541296	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E3151_RS18185	3541448..3541819	-	372	WP_000090264.1	antitermination protein	-
E3151_RS18190	3541809..3542180	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E3151_RS18195	3542193..3543242	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E3151_RS18200	3543244..3543522	-	279	WP_001341388.1	hypothetical protein	-
E3151_RS18205	3543690..3543845	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3543857..3543915	+	59	-	-	Antitoxin
E3151_RS18210	3543947..3544084	+	138	WP_000955173.1	hypothetical protein	-
E3151_RS18215	3544450..3545223	+	774	WP_000160654.1	alpha/beta hydrolase	-
E3151_RS18220	3545575..3545988	-	414	WP_001151233.1	DUF977 family protein	-
E3151_RS18225	3546004..3546774	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3151_RS18230	3546796..3547542	-	747	WP_000788751.1	ATP-binding protein	-
E3151_RS18235	3547549..3548640	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T122707 WP_000813263.1 NZ_CP038423:c3543845-3543690 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T122707 NZ_CP038423:c3543845-3543690 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122707 NZ_CP038423:3543857-3543915 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin