Detailed information of TA system TA122704

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2977028..2977253
• Replicon: chromosome
• Accession: NZ_CP038423
• Organism: Escherichia coli O157:H7 strain 7409

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3151_RS14980
• Protein ID: WP_000967408.1
• Coordinates: 2977028..2977240 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2977195..2977253 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3151_RS14945	2972331..2973389	-	1059	WP_000483497.1	site-specific DNA-methyltransferase	-
E3151_RS14950	2973540..2973737	-	198	WP_000917735.1	hypothetical protein	-
E3151_RS14955	2973964..2974785	-	822	WP_000762904.1	antitermination protein	-
E3151_RS14960	2974782..2975156	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E3151_RS14965	2975169..2976218	-	1050	Protein_2928	DUF968 domain-containing protein	-
E3151_RS14970	2976220..2976498	-	279	WP_001304183.1	hypothetical protein	-
E3151_RS14975	2976564..2976731	-	168	WP_000998188.1	hypothetical protein	-
E3151_RS14980	2977028..2977240	-	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2977195..2977253	+	59	-	-	Antitoxin
E3151_RS14985	2977429..2977533	-	105	WP_001278450.1	hypothetical protein	-
E3151_RS14990	2977649..2978011	-	363	WP_000610379.1	DUF551 domain-containing protein	-
E3151_RS14995	2978008..2978379	-	372	WP_000137941.1	hypothetical protein	-
E3151_RS15000	2978415..2978627	-	213	WP_000063625.1	hypothetical protein	-
E3151_RS15005	2978676..2979032	-	357	WP_001302146.1	hypothetical protein	-
E3151_RS15010	2979089..2979484	-	396	WP_001118161.1	DUF977 family protein	-
E3151_RS15015	2979500..2980270	-	771	WP_000450888.1	DUF1627 domain-containing protein	-
E3151_RS15020	2980300..2981040	-	741	WP_000790456.1	ATP-binding protein	-
E3151_RS15025	2981047..2982000	-	954	WP_000095667.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI / paa	2891927..3049928	158001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T122704 WP_000967408.1 NZ_CP038423:c2977240-2977028 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T122704 NZ_CP038423:c2977240-2977028 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122704 NZ_CP038423:2977195-2977253 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin