Detailed information of TA system TA122702

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2929041..2929266
• Replicon: chromosome
• Accession: NZ_CP038423
• Organism: Escherichia coli O157:H7 strain 7409

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3151_RS14670
• Protein ID: WP_000935259.1
• Coordinates: 2929041..2929253 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2929208..2929266 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3151_RS14620	2924044..2924475	-	432	WP_001302123.1	tellurite resistance protein	-
E3151_RS14635	2924926..2925639	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E3151_RS14640	2925775..2925972	-	198	WP_000917763.1	hypothetical protein	-
E3151_RS14645	2926197..2926751	-	555	WP_000640035.1	DUF1133 family protein	-
E3151_RS14650	2926814..2927119	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E3151_RS14655	2927132..2928181	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
E3151_RS14660	2928183..2928455	-	273	WP_000191872.1	hypothetical protein	-
E3151_RS14665	2928577..2928921	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E3151_RS14670	2929041..2929253	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2929208..2929266	+	59	-	-	Antitoxin
E3151_RS14675	2929487..2930044	-	558	WP_000104474.1	DUF551 domain-containing protein	-
E3151_RS14680	2930046..2930264	-	219	WP_000683609.1	DUF4014 family protein	-
E3151_RS14685	2930392..2930703	-	312	WP_001289673.1	hypothetical protein	-
E3151_RS14690	2930696..2930923	-	228	WP_000699809.1	hypothetical protein	-
E3151_RS14695	2930920..2931201	-	282	WP_000603384.1	DNA-binding protein	-
E3151_RS14700	2931234..2931950	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
E3151_RS14705	2931984..2932445	-	462	WP_000139447.1	replication protein	-
E3151_RS14710	2932438..2933481	-	1044	WP_001262402.1	hypothetical protein	-
E3151_RS14715	2933550..2933975	-	426	WP_000693878.1	Rha family transcriptional regulator	-
E3151_RS14720	2933959..2934201	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI / paa	2891927..3049928	158001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T122702 WP_000935259.1 NZ_CP038423:c2929253-2929041 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T122702 NZ_CP038423:c2929253-2929041 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122702 NZ_CP038423:2929208-2929266 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin