Detailed information of TA system TA122677

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4768523..4768935
• Replicon: chromosome
• Accession: NZ_CP038421
• Organism: Escherichia coli O157:H7 strain 7636

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: E3152_RS23705
• Protein ID: WP_000132630.1
• Coordinates: 4768594..4768935 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4768523..4768599 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3152_RS23695	4765150..4766619	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
E3152_RS23700	4766619..4768373	+	1755	WP_000110094.1	restriction endonuclease subunit S	-
-	4768523..4768599	-	77	NuclAT_14	-	Antitoxin
-	4768523..4768599	-	77	NuclAT_14	-	Antitoxin
-	4768523..4768599	-	77	NuclAT_14	-	Antitoxin
-	4768523..4768599	-	77	NuclAT_14	-	Antitoxin
-	4768523..4768599	-	77	NuclAT_15	-	Antitoxin
-	4768523..4768599	-	77	NuclAT_15	-	Antitoxin
-	4768523..4768599	-	77	NuclAT_15	-	Antitoxin
-	4768523..4768599	-	77	NuclAT_15	-	Antitoxin
E3152_RS23705	4768594..4768935	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
E3152_RS23710	4768982..4770145	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
E3152_RS23715	4770193..4771074	-	882	WP_001304007.1	DUF262 domain-containing protein	-
E3152_RS23720	4771171..4771335	-	165	WP_000394274.1	DUF1127 domain-containing protein	-
E3152_RS23725	4771512..4772924	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
E3152_RS23730	4773167..4773367	-	201	WP_001310455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T122677 WP_000132630.1 NZ_CP038421:4768594-4768935 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T122677 NZ_CP038421:4768594-4768935 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT122677 NZ_CP038421:c4768599-4768523 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin