Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4520032..4520571 Replicon chromosome
Accession NZ_CP038419
Organism Escherichia coli O157:H7 strain 2-6-2

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag E4U89_RS22915 Protein ID WP_001303998.1
Coordinates 4520311..4520571 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag E4U89_RS22910 Protein ID WP_000729705.1
Coordinates 4520032..4520292 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U89_RS22890 4515786..4516571 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E4U89_RS22895 4516516..4518255 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E4U89_RS22900 4518400..4518897 - 498 Protein_4490 REP-associated tyrosine transposase RayT -
E4U89_RS22905 4519073..4519846 - 774 WP_000543891.1 C40 family peptidase -
E4U89_RS22910 4520032..4520292 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E4U89_RS22915 4520311..4520571 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E4U89_RS22920 4520727..4521467 + 741 WP_001225679.1 murein L,D-transpeptidase -
E4U89_RS22925 4521438..4522205 - 768 WP_001301698.1 class II glutamine amidotransferase -
E4U89_RS22930 4522310..4522888 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T122638 WP_001303998.1 NZ_CP038419:4520311-4520571 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T122638 NZ_CP038419:4520311-4520571 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT122638 WP_000729705.1 NZ_CP038419:4520032-4520292 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT122638 NZ_CP038419:4520032-4520292 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References