Detailed information of TA system TA122631

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3530845..3531070
• Replicon: chromosome
• Accession: NZ_CP038419
• Organism: Escherichia coli O157:H7 strain 2-6-2

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U89_RS18185
• Protein ID: WP_000813263.1
• Coordinates: 3530845..3531000 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3531012..3531070 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U89_RS18150	3526299..3527012	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U89_RS18155	3527150..3527346	-	197	Protein_3558	TrmB family transcriptional regulator	-
E4U89_RS18160	3527633..3528451	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4U89_RS18165	3528603..3528974	-	372	WP_000090264.1	antitermination protein	-
E4U89_RS18170	3528964..3529335	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U89_RS18175	3529348..3530397	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U89_RS18180	3530399..3530677	-	279	WP_001341388.1	hypothetical protein	-
E4U89_RS18185	3530845..3531000	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3531012..3531070	+	59	-	-	Antitoxin
E4U89_RS18190	3531102..3531239	+	138	WP_000955173.1	hypothetical protein	-
E4U89_RS18195	3531605..3532378	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4U89_RS18200	3532730..3533143	-	414	WP_001151233.1	DUF977 family protein	-
E4U89_RS18205	3533159..3533929	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U89_RS18210	3533951..3534697	-	747	WP_000788751.1	ATP-binding protein	-
E4U89_RS18215	3534704..3535795	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T122631 WP_000813263.1 NZ_CP038419:c3531000-3530845 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T122631 NZ_CP038419:c3531000-3530845 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122631 NZ_CP038419:3531012-3531070 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin