Detailed information of TA system TA122628

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2952918..2953143
• Replicon: chromosome
• Accession: NZ_CP038419
• Organism: Escherichia coli O157:H7 strain 2-6-2

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U89_RS14820
• Protein ID: WP_000935259.1
• Coordinates: 2952918..2953130 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2953085..2953143 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U89_RS14770	2947921..2948352	-	432	WP_001302123.1	tellurite resistance protein	-
E4U89_RS14785	2948803..2949516	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U89_RS14790	2949652..2949849	-	198	WP_000917763.1	hypothetical protein	-
E4U89_RS14795	2950074..2950628	-	555	WP_000640035.1	DUF1133 family protein	-
E4U89_RS14800	2950691..2950996	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U89_RS14805	2951009..2952058	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U89_RS14810	2952060..2952332	-	273	WP_000191872.1	hypothetical protein	-
E4U89_RS14815	2952454..2952798	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U89_RS14820	2952918..2953130	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2953085..2953143	+	59	-	-	Antitoxin
E4U89_RS14825	2953364..2953921	-	558	WP_000104474.1	DUF551 domain-containing protein	-
E4U89_RS14830	2953923..2954141	-	219	WP_000683609.1	DUF4014 family protein	-
E4U89_RS14835	2954269..2954580	-	312	WP_001289673.1	hypothetical protein	-
E4U89_RS14840	2954573..2954800	-	228	WP_000699809.1	hypothetical protein	-
E4U89_RS14845	2954797..2955078	-	282	WP_000603384.1	DNA-binding protein	-
E4U89_RS14850	2955111..2955827	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U89_RS14855	2955861..2956322	-	462	WP_000139447.1	replication protein	-
E4U89_RS14860	2956315..2957358	-	1044	WP_001262402.1	hypothetical protein	-
E4U89_RS14865	2957427..2957852	-	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U89_RS14870	2957836..2958078	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa	2915806..2998831	83025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T122628 WP_000935259.1 NZ_CP038419:c2953130-2952918 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T122628 NZ_CP038419:c2953130-2952918 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122628 NZ_CP038419:2953085-2953143 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin