Detailed information of TA system TA122590

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3544462..3544687
• Replicon: chromosome
• Accession: NZ_CP038416
• Organism: Escherichia coli O157:H7 strain 3-5-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U90_RS18160
• Protein ID: WP_000813263.1
• Coordinates: 3544462..3544617 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3544629..3544687 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U90_RS18125	3539916..3540629	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U90_RS18130	3540767..3540963	-	197	Protein_3550	TrmB family transcriptional regulator	-
E4U90_RS18135	3541250..3542068	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4U90_RS18140	3542220..3542591	-	372	WP_000090264.1	antitermination protein	-
E4U90_RS18145	3542581..3542952	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U90_RS18150	3542965..3544014	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U90_RS18155	3544016..3544294	-	279	WP_001341388.1	hypothetical protein	-
E4U90_RS18160	3544462..3544617	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3544629..3544687	+	59	-	-	Antitoxin
E4U90_RS18165	3544719..3544856	+	138	WP_000955173.1	hypothetical protein	-
E4U90_RS18170	3545222..3545995	+	774	WP_000160650.1	alpha/beta hydrolase	-
E4U90_RS18175	3546347..3546760	-	414	WP_001151233.1	DUF977 family protein	-
E4U90_RS18180	3546776..3547546	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U90_RS18185	3547568..3548314	-	747	WP_000788745.1	ATP-binding protein	-
E4U90_RS18190	3548321..3549412	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T122590 WP_000813263.1 NZ_CP038416:c3544617-3544462 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T122590 NZ_CP038416:c3544617-3544462 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122590 NZ_CP038416:3544629-3544687 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin