Detailed information of TA system TA122586

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2637140..2637365
• Replicon: chromosome
• Accession: NZ_CP038416
• Organism: Escherichia coli O157:H7 strain 3-5-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U90_RS13385
• Protein ID: WP_000935259.1
• Coordinates: 2637153..2637365 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2637140..2637198 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U90_RS13335	2632192..2632435	+	244	Protein_2605	hypothetical protein	-
E4U90_RS13340	2632419..2632844	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U90_RS13345	2632913..2633968	+	1056	WP_001356791.1	hypothetical protein	-
E4U90_RS13350	2633961..2634422	+	462	WP_000139447.1	replication protein	-
E4U90_RS13355	2634456..2635172	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U90_RS13360	2635205..2635486	+	282	WP_000603384.1	DNA-binding protein	-
E4U90_RS13365	2635483..2635710	+	228	WP_000699809.1	hypothetical protein	-
E4U90_RS13370	2635703..2636014	+	312	WP_001289673.1	hypothetical protein	-
E4U90_RS13375	2636142..2636360	+	219	WP_000683609.1	DUF4014 family protein	-
E4U90_RS13380	2636362..2636919	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2637140..2637198	-	59	-	-	Antitoxin
E4U90_RS13385	2637153..2637365	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U90_RS13390	2637485..2637829	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U90_RS13395	2637951..2638223	+	273	WP_000191871.1	hypothetical protein	-
E4U90_RS13400	2638225..2639274	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U90_RS13405	2639287..2639592	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U90_RS13410	2639655..2640209	+	555	WP_000640035.1	DUF1133 family protein	-
E4U90_RS13415	2640434..2640631	+	198	WP_000917763.1	hypothetical protein	-
E4U90_RS13420	2640767..2641480	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U90_RS13435	2641931..2642362	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2576651..2674812	98161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T122586 WP_000935259.1 NZ_CP038416:2637153-2637365 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T122586 NZ_CP038416:2637153-2637365 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122586 NZ_CP038416:c2637198-2637140 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin