Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 716904..717703 Replicon chromosome
Accession NZ_CP038416
Organism Escherichia coli O157:H7 strain 3-5-1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag E4U90_RS03525 Protein ID WP_000347273.1
Coordinates 716904..717368 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag E4U90_RS03530 Protein ID WP_001302819.1
Coordinates 717368..717703 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U90_RS03495 711905..712339 - 435 WP_000948831.1 PTS sugar transporter subunit IIA -
E4U90_RS03500 712357..713235 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
E4U90_RS03505 713225..714004 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
E4U90_RS03510 714015..714488 - 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
E4U90_RS03515 714511..715791 - 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
E4U90_RS03520 716040..716849 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
E4U90_RS03525 716904..717368 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
E4U90_RS03530 717368..717703 - 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
E4U90_RS03535 717852..719423 - 1572 WP_001273776.1 galactarate dehydratase -
E4U90_RS03540 719798..721132 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
E4U90_RS03545 721148..721918 + 771 WP_001058231.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T122573 WP_000347273.1 NZ_CP038416:c717368-716904 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T122573 NZ_CP038416:c717368-716904 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT122573 WP_001302819.1 NZ_CP038416:c717703-717368 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT122573 NZ_CP038416:c717703-717368 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure

References