Detailed information of TA system TA122526

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68697..69118
• Replicon: plasmid p493-89-1
• Accession: NZ_CP038413
• Organism: Escherichia coli O157:H7 strain 493/89

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: E3172_RS27430
• Protein ID: WP_001302184.1
• Coordinates: 68697..68855 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	68920..69118 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3172_RS27385	63762..63977	-	216	WP_000086379.1	conjugal transfer relaxosome protein TraY	-
E3172_RS27390	64113..64760	-	648	WP_000332519.1	conjugal transfer transcriptional regulator TraJ	-
E3172_RS27395	64951..65334	-	384	WP_001063021.1	relaxosome protein TraM	-
E3172_RS27400	65685..66275	+	591	WP_168940513.1	transglycosylase SLT domain-containing protein	-
E3172_RS27405	66571..67392	-	822	WP_001234448.1	DUF945 domain-containing protein	-
E3172_RS27410	67512..67709	-	198	WP_045174704.1	hypothetical protein	-
E3172_RS27415	67862..68098	+	237	WP_086260624.1	hypothetical protein	-
E3172_RS27420	68034..68219	-	186	Protein_77	hypothetical protein	-
E3172_RS27425	68142..68459	+	318	WP_000820278.1	hypothetical protein	-
E3172_RS27430	68697..68855	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	68920..69118	-	199	NuclAT_0	-	Antitoxin
-	68920..69118	-	199	NuclAT_0	-	Antitoxin
-	68920..69118	-	199	NuclAT_0	-	Antitoxin
-	68920..69118	-	199	NuclAT_0	-	Antitoxin
E3172_RS27435	69130..69849	-	720	WP_001276249.1	plasmid SOS inhibition protein A	-
E3172_RS27440	69846..70280	-	435	WP_000845908.1	conjugation system SOS inhibitor PsiB	-
E3172_RS27445	70335..72293	-	1959	WP_000117168.1	ParB/RepB/Spo0J family partition protein	-
E3172_RS27450	72359..72592	-	234	WP_000005995.1	DUF905 family protein	-
E3172_RS27455	72649..73101	-	453	WP_000290823.1	single-stranded DNA-binding protein	-
E3172_RS27460	73127..73333	-	207	WP_000547965.1	hypothetical protein	-
E3172_RS27465	73403..73846	+	444	WP_001492078.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papC / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE	1..121214	121214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T122526 WP_001302184.1 NZ_CP038413:c68855-68697 [Escherichia coli O157:H7]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T122526 NZ_CP038413:c68855-68697 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT122526 NZ_CP038413:c69118-68920 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin