Detailed information of TA system TA122523

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30094..30347
• Replicon: plasmid p493-89-1
• Accession: NZ_CP038413
• Organism: Escherichia coli O157:H7 strain 493/89

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: E3172_RS27185
• Protein ID: WP_001312851.1
• Coordinates: 30094..30243 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	30289..30347 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3172_RS27170	28395..29252	-	858	WP_000130951.1	incFII family plasmid replication initiator RepA	-
E3172_RS27175	29245..29319	-	75	WP_001375168.1	RepA leader peptide Tap	-
E3172_RS27180	29562..29810	-	249	WP_000083848.1	replication regulatory protein RepA	-
E3172_RS27185	30094..30243	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	30289..30347	+	59	NuclAT_1	-	Antitoxin
-	30289..30347	+	59	NuclAT_1	-	Antitoxin
-	30289..30347	+	59	NuclAT_1	-	Antitoxin
-	30289..30347	+	59	NuclAT_1	-	Antitoxin
E3172_RS27190	30522..31112	-	591	WP_000766799.1	DUF2726 domain-containing protein	-
E3172_RS27195	31150..31363	-	214	Protein_32	transcriptional regulator	-
E3172_RS27200	31409..31870	-	462	WP_001233860.1	thermonuclease family protein	-
E3172_RS27205	32116..32328	-	213	WP_001299730.1	ANR family transcriptional regulator	-
E3172_RS27210	32462..33022	-	561	WP_000139383.1	fertility inhibition protein FinO	-
E3172_RS27215	33077..33823	-	747	WP_000205743.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papC / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE	1..121214	121214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T122523 WP_001312851.1 NZ_CP038413:c30243-30094 [Escherichia coli O157:H7]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T122523 NZ_CP038413:c30243-30094 [Escherichia coli O157:H7]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTTACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122523 NZ_CP038413:30289-30347 [Escherichia coli O157:H7]
AGGAACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin