Detailed information of TA system TA122516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4414489..4415183
• Replicon: chromosome
• Accession: NZ_CP038412
• Organism: Escherichia coli O157:H7 strain 493/89

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E3172_RS22100
• Protein ID: WP_001263495.1
• Coordinates: 4414489..4414887 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: C3TNY2
• Locus tag: E3172_RS22105
• Protein ID: WP_000554761.1
• Coordinates: 4414890..4415183 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4410076..4410156	-	81	NuclAT_12	-	-
-	4410076..4410156	-	81	NuclAT_12	-	-
-	4410076..4410156	-	81	NuclAT_12	-	-
-	4410076..4410156	-	81	NuclAT_12	-	-
E3172_RS22075	4410752..4411210	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E3172_RS22080	4411471..4412928	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E3172_RS22085	4412985..4413542	-	558	WP_023438539.1	peptide chain release factor H	-
E3172_RS22090	4413454..4413720	-	267	WP_001303804.1	hypothetical protein	-
E3172_RS22095	4414027..4414479	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E3172_RS22100	4414489..4414887	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E3172_RS22105	4414890..4415183	-	294	WP_000554761.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E3172_RS22110	4415235..4416290	-	1056	WP_001226186.1	DNA polymerase IV	-
E3172_RS22115	4416361..4417146	-	786	WP_000207562.1	putative lateral flagellar export/assembly protein LafU	-
E3172_RS22120	4417091..4418830	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E3172_RS22125	4418975..4419472	-	498	Protein_4340	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T122516 WP_001263495.1 NZ_CP038412:c4414887-4414489 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T122516 NZ_CP038412:c4414887-4414489 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11261.86 Da
• Isoelectric Point: 5.9050

>AT122516 WP_000554761.1 NZ_CP038412:c4415183-4414890 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLVEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT122516 NZ_CP038412:c4415183-4414890 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
TTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA