Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) ralRA/-
Location 2711385..2711756 Replicon chromosome
Accession NZ_CP038412
Organism Escherichia coli O157:H7 strain 493/89

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag E3172_RS13585 Protein ID WP_001317028.1
Coordinates 2711562..2711756 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2711385..2711563 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3172_RS13555 2707137..2707307 + 171 WP_001625136.1 hypothetical protein -
E3172_RS13560 2707340..2708713 + 1374 WP_000123747.1 ATP-dependent RNA helicase DbpA -
E3172_RS13565 2708842..2709777 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
E3172_RS13570 2709829..2711064 - 1236 WP_000040852.1 site-specific integrase -
E3172_RS13575 2711066..2711281 - 216 WP_000079604.1 excisionase XisR -
E3172_RS13580 2711360..2711569 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2711385..2711563 + 179 NuclAT_0 - Antitoxin
- 2711385..2711563 + 179 NuclAT_0 - Antitoxin
- 2711385..2711563 + 179 NuclAT_0 - Antitoxin
- 2711385..2711563 + 179 NuclAT_0 - Antitoxin
E3172_RS13585 2711562..2711756 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
E3172_RS13590 2711813..2712622 - 810 WP_000595430.1 recombination protein RecT -
E3172_RS13595 2712615..2715290 - 2676 WP_000105108.1 exodeoxyribonuclease VIII -
E3172_RS13600 2715392..2715668 - 277 Protein_2666 hypothetical protein -
E3172_RS13605 2715743..2715919 - 177 WP_000245528.1 hypothetical protein -
E3172_RS13610 2715913..2716134 - 222 WP_000560227.1 cell division protein FtsZ -
E3172_RS13615 2716543..2716695 - 153 WP_000379547.1 DUF1391 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2709829..2753484 43655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-54)


Sequences


Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T122498 WP_001317028.1 NZ_CP038412:c2711756-2711562 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T122498 NZ_CP038412:c2711756-2711562 [Escherichia coli O157:H7]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT122498 NZ_CP038412:2711385-2711563 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7


Antitoxin

Download structure file

References