Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4434571..4435110 Replicon chromosome
Accession NZ_CP038405
Organism Escherichia coli O157:H7 strain ATCC 35150

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag E3153_RS22365 Protein ID WP_001303998.1
Coordinates 4434850..4435110 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag E3153_RS22360 Protein ID WP_000729705.1
Coordinates 4434571..4434831 (+) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3153_RS22340 4430325..4431110 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E3153_RS22345 4431055..4432794 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E3153_RS22350 4432939..4433436 - 498 Protein_4378 REP-associated tyrosine transposase RayT -
E3153_RS22355 4433612..4434385 - 774 WP_000543891.1 C40 family peptidase -
E3153_RS22360 4434571..4434831 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E3153_RS22365 4434850..4435110 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E3153_RS22370 4435266..4436006 + 741 WP_001225679.1 murein L,D-transpeptidase -
E3153_RS22375 4435977..4436744 - 768 WP_001301698.1 class II glutamine amidotransferase -
E3153_RS22380 4436849..4437427 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-85)

Antitoxin

(1-85)


Sequences


Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T122450 WP_001303998.1 NZ_CP038405:4434850-4435110 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T122450 NZ_CP038405:4434850-4435110 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT122450 WP_000729705.1 NZ_CP038405:4434571-4434831 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT122450 NZ_CP038405:4434571-4434831 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure

References