Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3263717..3264512 | Replicon | chromosome |
| Accession | NZ_CP038405 | ||
| Organism | Escherichia coli O157:H7 strain ATCC 35150 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | E3153_RS16640 | Protein ID | WP_000854914.1 |
| Coordinates | 3263717..3264091 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0B0VS41 |
| Locus tag | E3153_RS16645 | Protein ID | WP_001280954.1 |
| Coordinates | 3264138..3264512 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E3153_RS16600 | 3258721..3259212 | - | 492 | WP_001301587.1 | DUF1097 domain-containing protein | - |
| E3153_RS16605 | 3259314..3259868 | - | 555 | WP_001001917.1 | molecular chaperone YcdY | - |
| E3153_RS16610 | 3259892..3260629 | - | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| E3153_RS16615 | 3260684..3261622 | - | 939 | WP_000351284.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| E3153_RS16625 | 3262093..3262935 | - | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| E3153_RS16630 | 3263020..3263217 | - | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| E3153_RS16635 | 3263229..3263720 | - | 492 | WP_000976853.1 | hypothetical protein | - |
| E3153_RS16640 | 3263717..3264091 | - | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| E3153_RS16645 | 3264138..3264512 | - | 375 | WP_001280954.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| E3153_RS16650 | 3264675..3264896 | - | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| E3153_RS16655 | 3264959..3265435 | - | 477 | WP_001186738.1 | RadC family protein | - |
| E3153_RS16660 | 3265451..3265936 | - | 486 | WP_000214398.1 | antirestriction protein | - |
| E3153_RS16665 | 3266027..3266845 | - | 819 | WP_001234682.1 | DUF945 domain-containing protein | - |
| E3153_RS16670 | 3267185..3268255 | - | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| E3153_RS16675 | 3268252..3269157 | - | 906 | WP_000203541.1 | chemotaxis protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | csgB / csgD / csgE / csgF / csgG | 3255103..3265936 | 10833 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T122445 WP_000854914.1 NZ_CP038405:c3264091-3263717 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T122445 NZ_CP038405:c3264091-3263717 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13718.51 Da Isoelectric Point: 6.6249
>AT122445 WP_001280954.1 NZ_CP038405:c3264512-3264138 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT122445 NZ_CP038405:c3264512-3264138 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0B0VS41 |