Detailed information of TA system TA122421

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4915258..4915670
• Replicon: chromosome
• Accession: NZ_CP038402
• Organism: Escherichia coli O157:H7 strain BB24-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: E4U87_RS24775
• Protein ID: WP_000132630.1
• Coordinates: 4915329..4915670 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4915258..4915334 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U87_RS24765	4911885..4913354	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
E4U87_RS24770	4913354..4915108	+	1755	WP_000110082.1	restriction endonuclease subunit S	-
-	4915258..4915334	-	77	NuclAT_14	-	Antitoxin
-	4915258..4915334	-	77	NuclAT_14	-	Antitoxin
-	4915258..4915334	-	77	NuclAT_14	-	Antitoxin
-	4915258..4915334	-	77	NuclAT_14	-	Antitoxin
-	4915258..4915334	-	77	NuclAT_15	-	Antitoxin
-	4915258..4915334	-	77	NuclAT_15	-	Antitoxin
-	4915258..4915334	-	77	NuclAT_15	-	Antitoxin
-	4915258..4915334	-	77	NuclAT_15	-	Antitoxin
E4U87_RS24775	4915329..4915670	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
E4U87_RS24780	4915717..4916880	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
E4U87_RS24785	4916928..4917809	-	882	WP_001304007.1	DUF262 domain-containing protein	-
E4U87_RS24790	4917906..4918070	-	165	WP_000394274.1	DUF1127 domain-containing protein	-
E4U87_RS24795	4918247..4919659	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
E4U87_RS24800	4919902..4920102	-	201	WP_001310455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002655 Escherichia coli O157:H7 str. EDL933 5456776..5475497 0.003 View NC_002695 Escherichia coli O157:H7 str. Sakai 5426911..5445631 0.003	inside	Genomic island	-	espX6	4907457..4928675	21218

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T122421 WP_000132630.1 NZ_CP038402:4915329-4915670 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T122421 NZ_CP038402:4915329-4915670 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT122421 NZ_CP038402:c4915334-4915258 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin