Detailed information of TA system TA122418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4568159..4568853
• Replicon: chromosome
• Accession: NZ_CP038402
• Organism: Escherichia coli O157:H7 strain BB24-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E4U87_RS23190
• Protein ID: WP_001263495.1
• Coordinates: 4568159..4568557 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E4U87_RS23195
• Protein ID: WP_000554757.1
• Coordinates: 4568560..4568853 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4563746..4563826	-	81	NuclAT_11	-	-
-	4563746..4563826	-	81	NuclAT_11	-	-
-	4563746..4563826	-	81	NuclAT_11	-	-
-	4563746..4563826	-	81	NuclAT_11	-	-
E4U87_RS23165	4564422..4564880	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E4U87_RS23170	4565141..4566598	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E4U87_RS23175	4566655..4567212	-	558	WP_023438539.1	peptide chain release factor H	-
E4U87_RS23180	4567124..4567390	-	267	WP_001303804.1	hypothetical protein	-
E4U87_RS23185	4567697..4568149	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E4U87_RS23190	4568159..4568557	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E4U87_RS23195	4568560..4568853	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E4U87_RS23200	4568905..4569960	-	1056	WP_001226186.1	DNA polymerase IV	-
E4U87_RS23205	4570031..4570816	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E4U87_RS23210	4570761..4572500	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E4U87_RS23215	4572645..4573142	-	498	Protein_4554	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4503720..4569960	66240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T122418 WP_001263495.1 NZ_CP038402:c4568557-4568159 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T122418 NZ_CP038402:c4568557-4568159 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT122418 WP_000554757.1 NZ_CP038402:c4568853-4568560 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT122418 NZ_CP038402:c4568853-4568560 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6