Detailed information of TA system TA122412

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3586382..3586607
• Replicon: chromosome
• Accession: NZ_CP038402
• Organism: Escherichia coli O157:H7 strain BB24-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U87_RS18510
• Protein ID: WP_000813263.1
• Coordinates: 3586382..3586537 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3586549..3586607 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U87_RS18475	3581836..3582549	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U87_RS18480	3582687..3582883	-	197	Protein_3623	TrmB family transcriptional regulator	-
E4U87_RS18485	3583170..3583988	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4U87_RS18490	3584140..3584511	-	372	WP_000090264.1	antitermination protein	-
E4U87_RS18495	3584501..3584872	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U87_RS18500	3584885..3585934	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U87_RS18505	3585936..3586214	-	279	WP_001341388.1	hypothetical protein	-
E4U87_RS18510	3586382..3586537	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3586549..3586607	+	59	-	-	Antitoxin
E4U87_RS18515	3586639..3586776	+	138	WP_000955173.1	hypothetical protein	-
E4U87_RS18520	3587142..3587915	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4U87_RS18525	3588267..3588680	-	414	WP_001151233.1	DUF977 family protein	-
E4U87_RS18530	3588696..3589466	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U87_RS18535	3589488..3590234	-	747	WP_000788751.1	ATP-binding protein	-
E4U87_RS18540	3590241..3591332	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T122412 WP_000813263.1 NZ_CP038402:c3586537-3586382 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T122412 NZ_CP038402:c3586537-3586382 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122412 NZ_CP038402:3586549-3586607 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin