Detailed information of TA system TA122409

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3050219..3050444
• Replicon: chromosome
• Accession: NZ_CP038402
• Organism: Escherichia coli O157:H7 strain BB24-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U87_RS15465
• Protein ID: WP_000935259.1
• Coordinates: 3050219..3050431 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3050386..3050444 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U87_RS15415	3045222..3045653	-	432	WP_001302123.1	tellurite resistance protein	-
E4U87_RS15430	3046104..3046817	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U87_RS15435	3046953..3047150	-	198	WP_000917763.1	hypothetical protein	-
E4U87_RS15440	3047375..3047929	-	555	WP_000640035.1	DUF1133 family protein	-
E4U87_RS15445	3047992..3048297	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U87_RS15450	3048310..3049359	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U87_RS15455	3049361..3049633	-	273	WP_000191872.1	hypothetical protein	-
E4U87_RS15460	3049755..3050099	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U87_RS15465	3050219..3050431	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3050386..3050444	+	59	-	-	Antitoxin
E4U87_RS15470	3050665..3051222	-	558	WP_000104474.1	DUF551 domain-containing protein	-
E4U87_RS15475	3051224..3051442	-	219	WP_000683609.1	DUF4014 family protein	-
E4U87_RS15480	3051570..3051881	-	312	WP_001289673.1	hypothetical protein	-
E4U87_RS15485	3051874..3052101	-	228	WP_000699809.1	hypothetical protein	-
E4U87_RS15490	3052098..3052379	-	282	WP_000603384.1	DNA-binding protein	-
E4U87_RS15495	3052412..3053128	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U87_RS15500	3053162..3053623	-	462	WP_000139447.1	replication protein	-
E4U87_RS15505	3053616..3054659	-	1044	WP_001262402.1	hypothetical protein	-
E4U87_RS15510	3054728..3055153	-	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U87_RS15515	3055137..3055379	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa	3013106..3096134	83028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T122409 WP_000935259.1 NZ_CP038402:c3050431-3050219 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T122409 NZ_CP038402:c3050431-3050219 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122409 NZ_CP038402:3050386-3050444 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin