Detailed information of TA system TA122406

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2687303..2687528
• Replicon: chromosome
• Accession: NZ_CP038402
• Organism: Escherichia coli O157:H7 strain BB24-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U87_RS13740
• Protein ID: WP_000967408.1
• Coordinates: 2687316..2687528 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2687303..2687361 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U87_RS13695	2682556..2683509	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
E4U87_RS13700	2683516..2684256	+	741	WP_000790456.1	ATP-binding protein	-
E4U87_RS13705	2684286..2685056	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
E4U87_RS13710	2685072..2685467	+	396	WP_001118161.1	DUF977 family protein	-
E4U87_RS13715	2685524..2685880	+	357	WP_001302146.1	hypothetical protein	-
E4U87_RS13720	2685929..2686141	+	213	WP_000063625.1	hypothetical protein	-
E4U87_RS13725	2686177..2686548	+	372	WP_000137941.1	hypothetical protein	-
E4U87_RS13730	2686545..2686907	+	363	WP_000610379.1	DUF551 domain-containing protein	-
E4U87_RS13735	2687023..2687127	+	105	WP_001278450.1	hypothetical protein	-
-	2687303..2687361	-	59	-	-	Antitoxin
E4U87_RS13740	2687316..2687528	+	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U87_RS13745	2687825..2687992	+	168	WP_000998188.1	hypothetical protein	-
E4U87_RS13750	2688058..2688336	+	279	WP_001304183.1	hypothetical protein	-
E4U87_RS13755	2688338..2689387	+	1050	Protein_2692	DUF968 domain-containing protein	-
E4U87_RS13760	2689400..2689774	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U87_RS13765	2689771..2690592	+	822	WP_000762904.1	antitermination protein	-
E4U87_RS13770	2690819..2691016	+	198	WP_000917735.1	hypothetical protein	-
E4U87_RS13775	2691167..2692225	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2607357..2719609	112252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T122406 WP_000967408.1 NZ_CP038402:2687316-2687528 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T122406 NZ_CP038402:2687316-2687528 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122406 NZ_CP038402:c2687361-2687303 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin