Detailed information of TA system TA122370

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3433552..3433777
• Replicon: chromosome
• Accession: NZ_CP038398
• Organism: Escherichia coli O157:H7 strain DEC4E

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3154_RS17355
• Protein ID: WP_000813263.1
• Coordinates: 3433552..3433707 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3433719..3433777 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3154_RS17320	3429006..3429719	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E3154_RS17325	3429857..3430053	-	197	Protein_3395	TrmB family transcriptional regulator	-
E3154_RS17330	3430340..3431158	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E3154_RS17335	3431310..3431681	-	372	WP_000090264.1	antitermination protein	-
E3154_RS17340	3431671..3432042	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E3154_RS17345	3432055..3433104	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E3154_RS17350	3433106..3433384	-	279	WP_001341388.1	hypothetical protein	-
E3154_RS17355	3433552..3433707	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3433719..3433777	+	59	-	-	Antitoxin
E3154_RS17360	3433809..3433946	+	138	WP_000955173.1	hypothetical protein	-
E3154_RS17365	3434312..3435085	+	774	WP_000160654.1	alpha/beta hydrolase	-
E3154_RS17370	3435437..3435850	-	414	WP_001151233.1	DUF977 family protein	-
E3154_RS17375	3435866..3436636	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3154_RS17380	3436658..3437404	-	747	WP_000788751.1	ATP-binding protein	-
E3154_RS17385	3437411..3438502	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T122370 WP_000813263.1 NZ_CP038398:c3433707-3433552 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T122370 NZ_CP038398:c3433707-3433552 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122370 NZ_CP038398:3433719-3433777 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin