Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2010607..2011109 Replicon chromosome
Accession NZ_CP038394
Organism Escherichia coli O157:H7 strain DEC5A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3155_RS09665 Protein ID WP_000767829.1
Coordinates 2010855..2011109 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3155_RS09660 Protein ID WP_001259255.1
Coordinates 2010607..2010858 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3155_RS09635 2005784..2006851 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3155_RS09640 2006851..2007921 - 1071 WP_000109000.1 histidinol-phosphate transaminase -
E3155_RS09645 2007918..2009222 - 1305 WP_001418019.1 histidinol dehydrogenase -
E3155_RS09650 2009228..2010127 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3155_RS09655 2010273..2010323 - 51 WP_013009236.1 his operon leader peptide -
E3155_RS09660 2010607..2010858 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3155_RS09665 2010855..2011109 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3155_RS09670 2011192..2012016 + 825 WP_000754737.1 SDR family oxidoreductase -
E3155_RS09675 2012062..2012991 + 930 WP_000803334.1 LysR family transcriptional regulator -
E3155_RS09680 2013206..2013268 + 63 WP_010723108.1 membrane protein YoeI -
E3155_RS09685 2013258..2014616 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3155_RS09690 2014795..2015853 + 1059 WP_000492327.1 transport protein YeeE -
E3155_RS09695 2015867..2016094 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T122324 WP_000767829.1 NZ_CP038394:2010855-2011109 [Escherichia coli O157:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T122324 NZ_CP038394:2010855-2011109 [Escherichia coli O157:H7]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT122324 WP_001259255.1 NZ_CP038394:2010607-2010858 [Escherichia coli O157:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT122324 NZ_CP038394:2010607-2010858 [Escherichia coli O157:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References