122324

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2010607..2011109 Replicon chromosome
Accession NZ_CP038394
Organism Escherichia coli O157:H7 strain DEC5A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3155_RS09665 Protein ID WP_000767829.1
Coordinates 2010855..2011109 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3155_RS09660 Protein ID WP_001259255.1
Coordinates 2010607..2010858 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3155_RS09635 2005784..2006851 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3155_RS09640 2006851..2007921 - 1071 WP_000109000.1 histidinol-phosphate transaminase -
E3155_RS09645 2007918..2009222 - 1305 WP_001418019.1 histidinol dehydrogenase -
E3155_RS09650 2009228..2010127 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3155_RS09655 2010273..2010323 - 51 WP_013009236.1 his operon leader peptide -
E3155_RS09660 2010607..2010858 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3155_RS09665 2010855..2011109 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3155_RS09670 2011192..2012016 + 825 WP_000754737.1 SDR family oxidoreductase -
E3155_RS09675 2012062..2012991 + 930 WP_000803334.1 LysR family transcriptional regulator -
E3155_RS09680 2013206..2013268 + 63 WP_010723108.1 membrane protein YoeI -
E3155_RS09685 2013258..2014616 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3155_RS09690 2014795..2015853 + 1059 WP_000492327.1 transport protein YeeE -
E3155_RS09695 2015867..2016094 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T122324 WP_000767829.1 NZ_CP038394:2010855-2011109 [Escherichia coli O157:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T122324 NZ_CP038394:2010855-2011109 [Escherichia coli O157:H7]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT122324 WP_001259255.1 NZ_CP038394:2010607-2010858 [Escherichia coli O157:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT122324 NZ_CP038394:2010607-2010858 [Escherichia coli O157:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References